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Literature summary for 1.1.1.34 extracted from
- Implication of HMGR in homeostasis of sequestered and de novo produced precursors of the iridoid biosynthesis in leaf beetle larvae (2008), Insect Biochem. Mol. Biol., 38, 76-88.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of the His-tagged catalytic domain of the enzyme, residues E416-F887, in Escherichia coli | Phaedon cochleariae |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 8-hydroxygeraniol | competitive inhibition mechanism, binds at the catalytic site, docking experiments, overview | Phaedon cochleariae |  |
| fluvastatin | - |
Phaedon cochleariae | |
| additional information | no inhibition by geraniol or by its glucoside and thioglucoside | Phaedon cochleariae |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | a transmembrane enzyme | Phaedon cochleariae | 16020 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 54500 | - |
x * 54500, recombinant His-tagged catalytic domain, SDS-PAGE | Phaedon cochleariae |
| 97100 | - |
2 * 97100, about, sequence calculation | Phaedon cochleariae |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| hydroxymethylglutaryl-CoA + NADPH + H+ | Phaedon cochleariae | HMGR is the key regulatory enzyme of the mevalonate pathway and also the iridoid biosynthesis, it is highly regulated itself, HMGR may represent a regulator in maintenance of homeostasis between de novo produced and sequestered intermediates of iridoid metabolism, overview | mevalonate + NADP+ + CoA | - |
? | |
| additional information | Phaedon cochleariae | HMGR activity is not only diminished in iridoid producers but most likely prevalent within the Chrysomelina subtribe and also within the insecta | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Phaedon cochleariae | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | the enzyme sequence contains a glycosylation site | Phaedon cochleariae |
| phosphoprotein | the enzyme sequence contains a phosphorylation site | Phaedon cochleariae |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged catalytic domain from Escherichia coli by nickel affinity chromatography | Phaedon cochleariae |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| larva | - |
Phaedon cochleariae | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| hydroxymethylglutaryl-CoA + NADPH + H+ | HMGR is the key regulatory enzyme of the mevalonate pathway and also the iridoid biosynthesis, it is highly regulated itself, HMGR may represent a regulator in maintenance of homeostasis between de novo produced and sequestered intermediates of iridoid metabolism, overview | Phaedon cochleariae | mevalonate + NADP+ + CoA | - |
? | |
| hydroxymethylglutaryl-CoA + NADPH + H+ | the enzyme utilizes two molecules of NADPH to mediate the four-electron reduction of HMG-CoA to the carboxylic acid mevalonate, homology modeling of the catalytic domain | Phaedon cochleariae | mevalonate + NADP+ + CoA | - |
? | |
| additional information | HMGR activity is not only diminished in iridoid producers but most likely prevalent within the Chrysomelina subtribe and also within the insecta | Phaedon cochleariae | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 54500, recombinant His-tagged catalytic domain, SDS-PAGE | Phaedon cochleariae |
| dimer | 2 * 97100, about, sequence calculation | Phaedon cochleariae |
| More | homology modeling of the catalytic domain | Phaedon cochleariae |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| HMGR | - |
Phaedon cochleariae |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
- |
Phaedon cochleariae |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | 35 | - |
Phaedon cochleariae |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
MOPS buffer | Phaedon cochleariae |
| 7.5 | - |
phosphate buffer | Phaedon cochleariae |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADPH | - |
Phaedon cochleariae | |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Phaedon cochleariae | sequence calculation | - |
5.9 |
IC50 Value
| IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|
| 0.00022 | - |
pH 6.8, 30°C | Phaedon cochleariae | fluvastatin | |
| 1.2 | - |
pH 6.8, 30°C, recombinant catalytic domain | Phaedon cochleariae | 8-hydroxygeraniol | |
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Release 2023.1 (January 2023)
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