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Literature summary for 1.1.1.34 extracted from

  • Hurtado-Guerrrero, R.; Pena-Diaz, J.; Montalvetti, A.; Ruiz-Perez, L.M.; Gonzalez-Pacanowska, D.
    Kinetic properties and inhibition of Trypanosoma cruzi 3-hydroxy-3-methylglutaryl CoA reductase (2002), FEBS Lett., 510, 141-144.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli strain BL21(DE3) as soluble protein Trypanosoma cruzi

Inhibitors

Inhibitors Comment Organism Structure
cerivastatin biphasic inhibition mechanism, slow, tight-binding type of inhibitor Trypanosoma cruzi
fluvastatin competitive Trypanosoma cruzi

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information kinetic analysis Trypanosoma cruzi
0.013
-
(S)-3-hydroxy-3-methylglutaryl-CoA pH 6.8, 25°C Trypanosoma cruzi
0.043
-
NADPH pH 6.8, 25°C Trypanosoma cruzi
0.074
-
CoA pH 8.3, 25°C Trypanosoma cruzi
0.22
-
NADP+ pH 8.3, 25°C Trypanosoma cruzi
0.252
-
(R)-mevalonate pH 8.3, 25°C Trypanosoma cruzi

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(S)-3-hydroxy-3-methylglutaryl-CoA + 2 NADPH + 2 H+ Trypanosoma cruzi
-
(R)-mevalonate + CoA + 2 NADP+
-
r

Organism

Organism UniProt Comment Textmining
Trypanosoma cruzi
-
-
-

Purification (Commentary)

Purification (Comment) Organism
3.8fold, recombinant enzyme from Escherichia coli Trypanosoma cruzi

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
28
-
purified recombinant enzyme Trypanosoma cruzi

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-3-hydroxy-3-methylglutaryl-CoA + 2 NADPH + 2 H+
-
Trypanosoma cruzi (R)-mevalonate + CoA + 2 NADP+
-
r
(S)-3-hydroxy-3-methylglutaryl-CoA + 2 NADPH + 2 H+ the reduction of 3-hydroxy-3-methylglutaryl-CoA is preferred over oxidation of mevalonate Trypanosoma cruzi (R)-mevalonate + CoA + 2 NADP+
-
r

Subunits

Subunits Comment Organism
tetramer
-
Trypanosoma cruzi

Synonyms

Synonyms Comment Organism
3-hydroxy-3-methylglutaryl CoA reductase
-
Trypanosoma cruzi
HMGR
-
Trypanosoma cruzi

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Trypanosoma cruzi

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.7 6.3 reduction of (S)-3-hydroxy-3-methylglutaryl-CoA Trypanosoma cruzi
8.7 9.6 oxidation of (R)-mevalonate Trypanosoma cruzi

Cofactor

Cofactor Comment Organism Structure
NADPH
-
Trypanosoma cruzi

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.027
-
fluvastatin pH 6.8, 25°C, versus (S)-3-hydroxy-3-methylglutaryl-CoA Trypanosoma cruzi