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Literature summary for 1.1.1.34 extracted from

  • Bischoff, K.M.; Rodwell, V.W.
    3-Hydroxy-3-methylglutaryl-coenzyme A reductase from Haloferax volcanii: Purification, characterization and expression in Escherichia coli (1996), J. Bacteriol., 178, 19-23.
    View publication on PubMedView publication on EuropePMC
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Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Haloferax volcanii

Inhibitors

Inhibitors Comment Organism Structure
(R)-3-hydroxy-3-methylglutaryl-CoA competitive inhibitor Haloferax volcanii
lovastatin
-
 Haloferax volcanii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.03
-
 CoA
-
 Haloferax volcanii
0.032
-
 NADPH reduction of R,S-mevaldehyde Haloferax volcanii
0.035
-
 NADP+
-
 Haloferax volcanii
0.06
-
 (S)-3-hydroxy-3-methylglutaryl-CoA
-
 Haloferax volcanii
0.066
-
 NADPH
-
 Haloferax volcanii
0.47
-
 (R,S)-mevaldehyde oxidative acylation of (R,S)-mevaldehyde Haloferax volcanii
0.55
-
 (R,S)-mevaldehyde reduction of (R,S)-mevaldehyde Haloferax volcanii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3-hydroxy-3-methylglutaryl-CoA + NADPH Haloferax volcanii
-
 (R)-mevalonate + CoA + NADP+
-
 r

Organism

Organism UniProt Comment Textmining
Haloferax volcanii
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Haloferax volcanii

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
24000
-
-
 Haloferax volcanii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(R,S)-mevaldehyde + acetate
-
 Haloferax volcanii ?
-
 ?
(R,S)-mevaldehyde + NADPH
-
 Haloferax volcanii ?
-
 ?
(S)-3-hydroxy-3-methylglutaryl-CoA + 2 NADPH + 2 H+
-
 Haloferax volcanii (R)-mevalonate + 2 NADP+ + CoA
-
 ?
3-hydroxy-3-methylglutaryl-CoA + NADPH
-
 Haloferax volcanii (R)-mevalonate + CoA + NADP+
-
 r

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
 reduction of (R,S)-mevaldehyde Haloferax volcanii
7.3
-
-
 Haloferax volcanii
8
-
 oxidative acylation of (R,S)-mevaldehyde Haloferax volcanii

Cofactor

Cofactor Comment Organism Structure
NADPH
-
 Haloferax volcanii