Literature summary for 1.1.1.332 extracted from

Wallwey, C.; Heddergott, C.; Xie, X.; Brakhage, A.A.; Li, S.M.
Genome mining reveals the presence of a conserved gene cluster for the biosynthesis of ergot alkaloid precursors in the fungal family Arthrodermataceae (2012), Microbiology, 158, 1634-1644.

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Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Trichophyton benhamiae

Inhibitors

Inhibitors	Comment	Organism	Structure
EDTA	5 mM, 71% of initial activity	Trichophyton benhamiae

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.09	-	chanoclavine-I	pH 7.5, 37°C	Trichophyton benhamiae
0.36	-	NAD+	pH 7.5, 37°C	Trichophyton benhamiae

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Co2+	5 mM, addition to EDTA-treated enzyme, 31% of control activity	Trichophyton benhamiae
Mn2+	5 mM, addition to EDTA-treated enzyme, 35% of control activity	Trichophyton benhamiae
Ni2+	5 mM, addition to EDTA-treated enzyme, 73% of control activity	Trichophyton benhamiae

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
26200	-	4 * 26200, calculated, 4 * 30000, SDS-PAGE of recombinant His-tagged protein	Trichophyton benhamiae
30000	-	4 * 26200, calculated, 4 * 30000, SDS-PAGE of recombinant His-tagged protein	Trichophyton benhamiae
128000	-	gel filtration	Trichophyton benhamiae

Organism

Organism	UniProt	Comment	Textmining
Trichophyton benhamiae	D4AK45	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
chanoclavine-I + NAD+	-	Trichophyton benhamiae	chanoclavine-I aldehyde + NADH + H+	-	?

Subunits

Subunits	Comment	Organism
tetramer	4 * 26200, calculated, 4 * 30000, SDS-PAGE of recombinant His-tagged protein	Trichophyton benhamiae

Synonyms

Synonyms	Comment	Organism
ARB_04646	-	Trichophyton benhamiae
ChaDH	-	Trichophyton benhamiae

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.76	-	chanoclavine-I	pH 7.5, 37°C	Trichophyton benhamiae

Cofactor

Cofactor	Comment	Organism	Structure
additional information	no cofactor: NADP+, FMN or FAD	Trichophyton benhamiae
NAD+	specific for NAD+	Trichophyton benhamiae

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Release 2023.1 (January 2023)