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Literature summary for 1.1.1.319 extracted from

  • Koeduka, T.; Fridman, E.; Gang, D.; Vassao, D.; Jackson, B.; Kish, C.; Orlova, I.; Spassova, S.; Lewis, N.; Noel, J.; Baiga, T.; Dudareva, N.; Pichersky, E.
    Eugenol and isoeugenol, characteristic aromatic constituents of spices, are biosynthesized via reduction of a coniferyl alcohol ester (2006), Proc. Natl. Acad. Sci. USA, 103, 10128-10133.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, quantitative expression analysis Petunia x hybrida

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
coniferyl acetate + NADPH + H+ Petunia x hybrida
-
isoeugenol + acetate + NADP+
-
?

Organism

Organism UniProt Comment Textmining
Petunia x hybrida Q15GI3 cv. Mitchell
-

Reaction

Reaction Comment Organism Reaction ID
isoeugenol + acetate + NADP+ = coniferyl acetate + NADPH + H+ reaction mechanism, overview Petunia x hybrida

Source Tissue

Source Tissue Comment Organism Textmining
flower
-
Petunia x hybrida
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
coniferyl acetate + NADPH + H+
-
Petunia x hybrida isoeugenol + acetate + NADP+
-
?
coniferyl acetate + NADPH + H+
-
Petunia x hybrida isoeugenol + acetate + NADP+ GC-MS analysis product analysis ?

Synonyms

Synonyms Comment Organism
IGS
-
Petunia x hybrida
IGS1
-
Petunia x hybrida
isoeugenol synthase 1
-
Petunia x hybrida

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
28
-
assay at Petunia x hybrida

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
assay at Petunia x hybrida

Cofactor

Cofactor Comment Organism Structure
NADPH
-
Petunia x hybrida

General Information

General Information Comment Organism
evolution the phenylpropene-forming isoeugenol, IGS, synthase belongs to a structural family of NADPH-dependent reductases that also includes eugenol synthase, EGS, pinoresinol–lariciresinol reductase, isoflavone reductase, and phenylcoumaran benzylic ether reductase, evolution and function of EGS1 and IGS1, overview Petunia x hybrida