Any feedback?
Literature summary for 1.1.1.319 extracted from
- The multiple phenylpropene synthases in both Clarkia breweri and Petunia hybrida represent two distinct protein lineages (2008), Plant J., 54, 362-374.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis | Petunia x hybrida |
| DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, expression in Escherichia coli | Clarkia breweri |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| L91I/L92H/D95N | site-directed mutagenesis, the mutant plants show increased eugenol production and an altered eugenol/isoeugenol ratio compared to the wild-type enzyme | Clarkia breweri |
| R73K/V77I/S83P/V84F | site-directed mutagenesis, the mutant plants show increased eugenol production and an altered eugenol/isoeugenol ratio compared to the wild-type enzyme | Clarkia breweri |
| R73K/V77I/S83P/V84F/Y87I/P88S/L91I/L92H/D95N | site-directed mutagenesis, the mutant plants show increased eugenol production and an altered eugenol/isoeugenol ratio compared to the wild-type enzyme | Clarkia breweri |
| S83P/V84F | site-directed mutagenesis, the mutant plants show increased eugenol production and an altered eugenol/isoeugenol ratio compared to the wild-type enzyme | Clarkia breweri |
| S83P/V84F/Y87I/P88S | site-directed mutagenesis, the mutant plants show increased eugenol production and an altered eugenol/isoeugenol ratio compared to the wild-type enzyme | Clarkia breweri |
| V84F | site-directed mutagenesis, the mutant plants show increased eugenol production and an altered eugenol/isoeugenol ratio compared to the wild-type enzyme | Clarkia breweri |
| V84F/Y87I | site-directed mutagenesis, the mutant plants show increased eugenol production and an altered eugenol/isoeugenol ratio compared to the wild-type enzyme | Clarkia breweri |
| V88F/Y91I | site-directed mutagenesis, the mutant plants show increased eugenol production and an altered eugenol/isoeugenol ratio compared to the wild-type enzyme | Petunia x hybrida |
| Y87I | site-directed mutagenesis, the mutant plants show increased eugenol production and an altered eugenol/isoeugenol ratio compared to the wild-type enzyme | Clarkia breweri |
| Y87I/P88S | site-directed mutagenesis, the mutant plants show increased eugenol production and an altered eugenol/isoeugenol ratio compared to the wild-type enzyme | Clarkia breweri |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.2115 | - |
coniferyl acetate | CbIGS1, pH 6.5, 28°C | Clarkia breweri |  |
| 0.2261 | - |
coniferyl acetate | PhIGS1, pH 6.5, 28°C | Petunia x hybrida | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| coniferyl acetate + NADPH + H+ | Petunia x hybrida | - |
isoeugenol + acetate + NADP+ | - |
? | |
| coniferyl acetate + NADPH + H+ | Clarkia breweri | - |
isoeugenol + acetate + NADP+ | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Clarkia breweri | - |
- |
- |
| Petunia x hybrida | - |
cv. Mitchell | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme 9.2fold from petals by alternating steps of anion exchange and hydrophobic interaction chromatography, co-purification with eugenol synthase, EC 1.1.1.318. Recombinant His-tagged CbIGS from Escherichia coli by nickel affinity chromatography | Clarkia breweri |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| flower | - |
Petunia x hybrida | - |
| flower | the highest specific activity levels of eugenol synthase are found in the stamens, followed closely by the pistil and petals | Clarkia breweri | - |
| petal | - |
Clarkia breweri | - |
| pistil | - |
Clarkia breweri | - |
| stamen | - |
Clarkia breweri | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.65 | - |
purified native, wild-type CbIGS1, pH 6.5, 28°C | Clarkia breweri |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| coniferyl acetate + NADPH + H+ | - |
Petunia x hybrida | isoeugenol + acetate + NADP+ | - |
? | |
| coniferyl acetate + NADPH + H+ | - |
Clarkia breweri | isoeugenol + acetate + NADP+ | - |
? | |
| coniferyl acetate + NADPH + H+ | CbIGS1 is specific for isoeugenol production | Clarkia breweri | isoeugenol + acetate + NADP+ | - |
? | |
| coniferyl acetate + NADPH + H+ | PhIGS1 uses a quinone methide intermediate-based mechanism to generate an intermediate to which the reductive transfer of the reducing hydride can then be easily accomplished | Petunia x hybrida | isoeugenol + acetate + NADP+ | - |
? | |
| additional information | the position of the substrate in the active site of PhIGS1 is such that the hydride is transferred to C9 instead of C7, as occurs in Ocimum basilicum eugenol synthase, EC 1.1.1.318 | Petunia x hybrida | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CbIGS1 | - |
Clarkia breweri |
| PhIGS1 | - |
Petunia x hybrida |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 28 | - |
assay at | Petunia x hybrida |
| 28 | - |
assay at | Clarkia breweri |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.99 | - |
coniferyl acetate | CbIGS1, pH 6.5, 28°C | Clarkia breweri | |
| 1.3 | - |
coniferyl acetate | PhIGS1, pH 6.5, 28°C | Petunia x hybrida | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
assay at | Petunia x hybrida |
| 6.5 | - |
assay at | Clarkia breweri |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADPH | - |
Petunia x hybrida | |
| NADPH | - |
Clarkia breweri | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | plants synthesize the volatile phenylpropene compounds eugenol and isoeugenol to serve in defense against herbivores and pathogens and to attract pollinators. Clarkia breweri flowers emit a mixture of eugenol and isoeugenol. Eugenol and isoeugenol differ in the position of the double bond in the propene side chain | Clarkia breweri |
| physiological function | plants synthesize the volatile phenylpropene compounds eugenol and isoeugenol to serve in defense against herbivores and pathogens and to attract pollinators. Petunia hybrida flowers emit mostly isoeugenol with small amounts of eugenol. Eugenol and isoeugenol differ in the position of the double bond in the propene side chain | Petunia x hybrida |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 4.7 | - |
coniferyl acetate | CbIGS1, pH 6.5, 28°C | Clarkia breweri | |
| 5.7 | - |
coniferyl acetate | PhIGS1, pH 6.5, 28°C | Petunia x hybrida | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
