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Literature summary for 1.1.1.319 extracted from

  • Koeduka, T.; Baiga, T.J.; Noel, J.P.; Pichersky, E.
    Biosynthesis of t-anethole in anise: characterization of t-anol/isoeugenol synthase and an O-methyltransferase specific for a C7-C8 propenyl side chain (2009), Plant Physiol., 149, 384-394.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.1.1.319

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination and analysis, phylogenetic analysis and tree, expression in Escherichia coli Pimpinella anisum

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ weak inhibition at 2.5 mM Pimpinella anisum
Cu2+ strong inhibition at 2.5 mM Pimpinella anisum
Fe2+ strong inhibition at 2.5 mM Pimpinella anisum
K+ weak inhibition at 2.5 mM Pimpinella anisum
Mn2+ weak inhibition at 2.5 mM Pimpinella anisum
additional information no inhibitory effect on the activity with 2.5 mM Na+ or Mg2+ Pimpinella anisum
Zn2+ strong inhibition at 2.5 mM Pimpinella anisum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.134
-
 4-coumaryl acetate pH 7.5, 25°C Pimpinella anisum
0.23
-
 coniferyl acetate pH 7.5, 25°C Pimpinella anisum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4-coumaryl acetate + NADPH + H+ Pimpinella anisum
-
 t-anol + acetate + NADP+
-
 ?
coniferyl acetate + NADPH + H+ Pimpinella anisum
-
 isoeugenol + acetate + NADP+
-
 ?

Organism

Organism UniProt Comment Textmining
Pimpinella anisum B8RCD2
-
-

Source Tissue

Source Tissue Comment Organism Textmining
fruit developing Pimpinella anisum
-
additional information the gene encoding AIS1 is expressed throughout the plant, its transcript level is highest in developing fruits Pimpinella anisum
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-coumaryl acetate + NADPH + H+
-
 Pimpinella anisum t-anol + acetate + NADP+
-
 ?
coniferyl acetate + NADPH + H+
-
 Pimpinella anisum isoeugenol + acetate + NADP+
-
 ?

Synonyms

Synonyms Comment Organism
AIS1
-
 Pimpinella anisum
IGS1
-
 Pimpinella anisum
PaAIS1
-
 Pimpinella anisum
t-anol/isoeugenol synthase 1
-
 Pimpinella anisum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Pimpinella anisum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
20
-
 purified recombinant enzyme, 30 min, stable Pimpinella anisum
37
-
 purified recombinant enzyme, 30 min, 85% activity remaining Pimpinella anisum
50
-
 purified recombinant enzyme, 30 min, nelow 45 activity remaining Pimpinella anisum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.07
-
 4-coumaryl acetate pH 7.5, 25°C Pimpinella anisum
1.02
-
 coniferyl acetate pH 7.5, 25°C Pimpinella anisum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Pimpinella anisum

Cofactor

Cofactor Comment Organism Structure
NADPH dependent on Pimpinella anisum

General Information

General Information Comment Organism
physiological function t-anol/isoeugenol synthase is an NADPH-dependent enzyme that can biosynthesize t-anol and isoeugenol from coumaryl acetate and coniferyl acetate, respectively Pimpinella anisum

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.52
-
 4-coumaryl acetate pH 7.5, 25°C Pimpinella anisum
4.44
-
 coniferyl acetate pH 7.5, 25°C Pimpinella anisum