Literature summary for 1.1.1.318 extracted from

Vassao, D.; Kim, S.; Milhollan, J.; Eichinger, D.; Davin, L.; Lewis, N.
A pinoresinol-lariciresinol reductase homologue from the creosote bush (Larrea tridentata) catalyzes the efficient in vitro conversion of p-coumaryl/coniferyl alcohol esters into the allylphenols chavicol/eugenol, but not the propenylphenols p-anol/isoeug (2007), Arch. Biochem. Biophys., 465, 209-218.

Search for more literature for 1.1.1.318

Cloned(Commentary)

Cloned (Comment)	Organism
DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression in GST-tagged Escherichia coli strain BL21	Larrea tridentata

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.21	-	4-coumaryl acetate	pH 7.5, 30°C, recombinant enzyme	Larrea tridentata
0.29	-	coniferyl acetate	pH 7.5, 30°C, recombinant enzyme	Larrea tridentata

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
4-coumaryl acetate + NADPH + H+	Larrea tridentata	-	chavicol + acetate + NADP+	-	?
coniferyl acetate + NADPH + H+	Larrea tridentata	-	eugenol + acetate + NADP+	-	?

Organism

Organism	UniProt	Comment	Textmining
Larrea tridentata	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant GST-taged enzyme from Escherichia coli strain BL21 by glutathione affinity chromatography and dialysis	Larrea tridentata

Reaction

Reaction	Comment	Organism	Reaction ID
eugenol + a carboxylate + NADP+ = a coniferyl ester + NADPH + H+	reaction mechanism, detailed overview	Larrea tridentata	a

Source Tissue

Source Tissue	Comment	Organism	Textmining
leaf	-	Larrea tridentata	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
4-coumaryl acetate + NADPH + H+	-	Larrea tridentata	chavicol + acetate + NADP+	-	?
4-coumaryl acetate + NADPH + H+	-	Larrea tridentata	chavicol + acetate + NADP+	GC-MS analysis product analysis	?
coniferyl acetate + NADPH + H+	-	Larrea tridentata	eugenol + acetate + NADP+	-	?
coniferyl acetate + NADPH + H+	-	Larrea tridentata	eugenol + acetate + NADP+	GC-MS analysis product analysis	?

Synonyms

Synonyms	Comment	Organism
chavicol/eugenol synthase	-	Larrea tridentata
LtCES1	-	Larrea tridentata

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
30	-	assay at	Larrea tridentata

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
6.46	-	coniferyl acetate	pH 7.5, 30°C, recombinant enzyme	Larrea tridentata
6.8	-	4-coumaryl acetate	pH 7.5, 30°C, recombinant enzyme	Larrea tridentata

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.5	-	assay at	Larrea tridentata

Cofactor

Cofactor	Comment	Organism	Structure
NADPH	-	Larrea tridentata

General Information

General Information	Comment	Organism
additional information	the enzyme is a PIP reductase homologue	Larrea tridentata

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
19500	-	4-coumaryl acetate	pH 7.5, 30°C, recombinant enzyme	Larrea tridentata
22000	-	coniferyl acetate	pH 7.5, 30°C, recombinant enzyme	Larrea tridentata

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Release 2023.1 (January 2023)