Cloned (Comment) | Organism |
---|---|
heterologous expression of a triple mutant (K98A/K242A/K294A) of perakine reductase in Escherichia coli | Rauvolfia serpentina |
Crystallization (Comment) | Organism |
---|---|
crystals of the purified and methylated enzyme are obtained by the hanging-drop vapour diffusion technique at 20°C with 100 mM sodium citrate pH 5.6 and 27% PEG 4000 as precipitant. Crystals belong to space group C2221 and diffract to 2.0 A, with unit-cell parameters a = 58.9, b = 93.0, c = 143.4 A | Rauvolfia serpentina |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rauvolfia serpentina | Q3L181 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Rauvolfia serpentina |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | probable dual functionality of perakine reductase, which may not only include alkaloid biosynthesis but may also include function in phenylpropanoid metabolism, generating cinnamic alcohols | Rauvolfia serpentina | ? | - |
? |