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Literature summary for 1.1.1.31 extracted from

  • Lee, P.; Raj, S.; Zhou, S.; Ashok, S.; Edwardraja, S.; Park, S.
    3-Hydroxyisobutyrate dehydrogenase-I from Pseudomonas denitrificans ATCC 13867 degrades 3-hydroxypropionic acid (2014), Biotechnol. Bioprocess Eng., 19, 1-7.
No PubMed abstract available
Search for more literature for 1.1.1.31

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol about 1575.34% activity at 1 mM Pseudomonas denitrificans (nom. rej.)
dithiothreitol about 182.11% activity at 1 mM Pseudomonas denitrificans (nom. rej.)
EDTA about 156.21% activity at 1 mM Pseudomonas denitrificans (nom. rej.)

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Pseudomonas denitrificans (nom. rej.)

Inhibitors

Inhibitors Comment Organism Structure
Ag+ complete inhibition at 1 mM Pseudomonas denitrificans (nom. rej.)
Co2+ about 68.1% residual activity at 1 mM Pseudomonas denitrificans (nom. rej.)
Cu2+ about 15.67% residual activity at 1 mM Pseudomonas denitrificans (nom. rej.)
Fe2+ complete inhibition at 1 mM Pseudomonas denitrificans (nom. rej.)
Hg2+ complete inhibition at 1 mM Pseudomonas denitrificans (nom. rej.)
K+ about 92.11% residual activity at 1 mM Pseudomonas denitrificans (nom. rej.)
Li+ about 92.32% residual activity at 1 mM Pseudomonas denitrificans (nom. rej.)
Mg2+ about 87.34% residual activity at 1 mM Pseudomonas denitrificans (nom. rej.)
NH4+ about 90.17% residual activity at 1 mM Pseudomonas denitrificans (nom. rej.)
Ni2+ about 63.67% residual activity at 1 mM Pseudomonas denitrificans (nom. rej.)
Zn2+ about 16.13% residual activity at 1 mM Pseudomonas denitrificans (nom. rej.)

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.12
-
 (S)-3-hydroxyisobutyrate at 37°C and pH 9.0 Pseudomonas denitrificans (nom. rej.)
2.09
-
 3-hydroxypropionic acid at 37°C and pH 9.0 Pseudomonas denitrificans (nom. rej.)
10.37
-
 methyl DL-serine at 37°C and pH 9.0 Pseudomonas denitrificans (nom. rej.)
10.48
-
 methyl (2S)-3-hydroxy-2-methylpropanoate at 37°C and pH 9.0 Pseudomonas denitrificans (nom. rej.)
31.84
-
 L-serine at 37°C and pH 9.0 Pseudomonas denitrificans (nom. rej.)

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ about 127.2% activity at 1 mM Pseudomonas denitrificans (nom. rej.)
additional information not influenced by Na+ Pseudomonas denitrificans (nom. rej.)

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
25000
-
 x * 25000, SDS-PAGE Pseudomonas denitrificans (nom. rej.)

Organism

Organism UniProt Comment Textmining
Pseudomonas denitrificans (nom. rej.)
-
-
-
Pseudomonas denitrificans (nom. rej.) ATCC 13867
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography and Superdex 200 gel filtration Pseudomonas denitrificans (nom. rej.)

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.62
-
 crude extract, at 37°C and pH 9.0 Pseudomonas denitrificans (nom. rej.)
58.67
-
 after 36.22fold purification, at 37°C and pH 9.0 Pseudomonas denitrificans (nom. rej.)

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-3-hydroxyisobutyrate + NAD+ the enzyme is very specific to (S)-3-hydroxyisobutyrate Pseudomonas denitrificans (nom. rej.) 2-methyl-3-oxopropanoate + NADH + H+
-
 ?
(S)-3-hydroxyisobutyrate + NAD+ the enzyme is very specific to (S)-3-hydroxyisobutyrate Pseudomonas denitrificans (nom. rej.) ATCC 13867 2-methyl-3-oxopropanoate + NADH + H+
-
 ?
3-hydroxypropionic acid + NAD+
-
 Pseudomonas denitrificans (nom. rej.) malonate semialdehyde + NADH + H+
-
 r
3-hydroxypropionic acid + NAD+
-
 Pseudomonas denitrificans (nom. rej.) ATCC 13867 malonate semialdehyde + NADH + H+
-
 r
L-serine + NAD+
-
 Pseudomonas denitrificans (nom. rej.) 3-oxo-L-alanine + NADH + H+
-
 r
L-serine + NAD+
-
 Pseudomonas denitrificans (nom. rej.) ATCC 13867 3-oxo-L-alanine + NADH + H+
-
 r
methyl (2S)-3-hydroxy-2-methylpropanoate + NAD+
-
 Pseudomonas denitrificans (nom. rej.) methyl (2S)-2-methyl-3-oxopropanoate + NADH + H+
-
 r
methyl (2S)-3-hydroxy-2-methylpropanoate + NAD+
-
 Pseudomonas denitrificans (nom. rej.) ATCC 13867 methyl (2S)-2-methyl-3-oxopropanoate + NADH + H+
-
 r
methyl DL-serine + NAD+
-
 Pseudomonas denitrificans (nom. rej.) methyl 3-oxo-DL-alanine + NADH + H+
-
 r
additional information no activity with (R)-3-hydroxyisobutyrate Pseudomonas denitrificans (nom. rej.) ?
-
 ?
additional information no activity with (R)-3-hydroxyisobutyrate Pseudomonas denitrificans (nom. rej.) ATCC 13867 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 25000, SDS-PAGE Pseudomonas denitrificans (nom. rej.)

Synonyms

Synonyms Comment Organism
3-hydroxyisobutyrate dehydrogenase-I
-
 Pseudomonas denitrificans (nom. rej.)
3HIBDHI
-
 Pseudomonas denitrificans (nom. rej.)

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
 Pseudomonas denitrificans (nom. rej.)

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 45 more than 50% activity between 30 and 40°C. Approximately 80% of the activity is lost at 45°C Pseudomonas denitrificans (nom. rej.)

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
7.94
-
 methyl DL-serine at 37°C and pH 9.0 Pseudomonas denitrificans (nom. rej.)
32.17
-
 3-hydroxypropionic acid at 37°C and pH 9.0 Pseudomonas denitrificans (nom. rej.)
41.35
-
 methyl (2S)-3-hydroxy-2-methylpropanoate at 37°C and pH 9.0 Pseudomonas denitrificans (nom. rej.)
47.16
-
 L-serine at 37°C and pH 9.0 Pseudomonas denitrificans (nom. rej.)
70.81
-
 (S)-3-hydroxyisobutyrate at 37°C and pH 9.0 Pseudomonas denitrificans (nom. rej.)

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
-
 Pseudomonas denitrificans (nom. rej.)

pH Range

pH Minimum pH Maximum Comment Organism
8 10 approximately 40% of the activity is retained at pH 11.0. At pH 7.0, the activity is reduced to 30% of the activity observed at pH 9.0 Pseudomonas denitrificans (nom. rej.)

Cofactor

Cofactor Comment Organism Structure
NAD+ the enzyme prefers NAD+ over NADP+ as a cofactor Pseudomonas denitrificans (nom. rej.)

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.75
-
 methyl DL-serine at 37°C and pH 9.0 Pseudomonas denitrificans (nom. rej.)
1.46
-
 L-serine at 37°C and pH 9.0 Pseudomonas denitrificans (nom. rej.)
4.04
-
 methyl (2S)-3-hydroxy-2-methylpropanoate at 37°C and pH 9.0 Pseudomonas denitrificans (nom. rej.)
15.4
-
 3-hydroxypropionic acid at 37°C and pH 9.0 Pseudomonas denitrificans (nom. rej.)
604.1
-
 (S)-3-hydroxyisobutyrate at 37°C and pH 9.0 Pseudomonas denitrificans (nom. rej.)