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Literature summary for 1.1.1.31 extracted from

  • Chowdhury, E.K.; Nagata, S.; Misono, H.
    3-Hydroxyisobutyrate dehydrogenase from Pseudomonas putida E23: purification and characterization (1996), Biosci. Biotechnol. Biochem., 60, 2043-2047.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
AgNO3
-
Diutina rugosa
AgNO3
-
Oryctolagus cuniculus
AgNO3
-
Pseudomonas putida
HgCl2
-
Pseudomonas putida
p-chloromercuribenzoate
-
Diutina rugosa
p-chloromercuribenzoate
-
Oryctolagus cuniculus
p-chloromercuribenzoate
-
Pseudomonas putida

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.023
-
NAD+
-
Oryctolagus cuniculus
0.05
-
NAD+
-
Diutina rugosa
0.061
-
L-3-hydroxyisobutyrate
-
Oryctolagus cuniculus
0.12
-
L-3-hydroxyisobutyrate
-
Pseudomonas putida
0.37
-
L-3-hydroxyisobutyrate
-
Diutina rugosa
0.44
-
NAD+
-
Pseudomonas putida
1.25
-
D-3-hydroxyisobutyrate
-
Diutina rugosa
18
-
3-hydroxypropionate
-
Pseudomonas putida
44
-
L-serine
-
Pseudomonas putida
83
-
2-methyl-D,L-serine
-
Pseudomonas putida

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30000
-
alpha4, 4 * 30000, SDS-PAGE Pseudomonas putida
74000
-
-
Oryctolagus cuniculus
80000
-
-
Diutina rugosa
120000
-
gel filtration Pseudomonas putida

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-3-hydroxyisobutyrate + NAD+ Oryctolagus cuniculus
-
L-2-methyl-3-oxopropionate + NADH
-
r
L-3-hydroxyisobutyrate + NAD+ Pseudomonas putida
-
L-2-methyl-3-oxopropionate + NADH
-
?
L-3-hydroxyisobutyrate + NAD+ Pseudomonas putida E23
-
L-2-methyl-3-oxopropionate + NADH
-
?

Organism

Organism UniProt Comment Textmining
Diutina rugosa
-
-
-
Oryctolagus cuniculus
-
-
-
Pseudomonas putida
-
E23
-
Pseudomonas putida E23
-
E23
-

Purification (Commentary)

Purification (Comment) Organism
-
Pseudomonas putida

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Oryctolagus cuniculus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
36.1
-
-
Diutina rugosa
294
-
-
Pseudomonas putida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-methyl-D,L-serine + NAD+
-
Pseudomonas putida ?
-
?
2-methyl-D,L-serine + NAD+
-
Pseudomonas putida E23 ?
-
?
3-hydroxypropanoate + NAD+
-
Oryctolagus cuniculus ?
-
?
3-hydroxypropanoate + NAD+
-
Pseudomonas putida ?
-
?
3-hydroxypropanoate + NAD+
-
Pseudomonas putida E23 ?
-
?
D-3-hydroxyisobutyrate + NAD+
-
Diutina rugosa methylmalonate semialdehyde + NADH + H+
-
r
L-3-hydroxyisobutyrate + NAD+
-
Oryctolagus cuniculus L-2-methyl-3-oxopropionate + NADH
-
r
L-3-hydroxyisobutyrate + NAD+
-
Pseudomonas putida L-2-methyl-3-oxopropionate + NADH
-
?
L-3-hydroxyisobutyrate + NAD+
-
Pseudomonas putida E23 L-2-methyl-3-oxopropionate + NADH
-
?
L-3-hydroxyisobutyrate + NAD+
-
Diutina rugosa methylmalonate semialdehyde + NADH + H+
-
r
L-serine + NAD+
-
Pseudomonas putida ?
-
?
L-serine + NAD+
-
Pseudomonas putida E23 ?
-
?

Subunits

Subunits Comment Organism
homodimer
-
Oryctolagus cuniculus
homodimer
-
Diutina rugosa
tetramer alpha4, 4 * 30000, SDS-PAGE Pseudomonas putida

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9 10.5
-
Diutina rugosa
9 11
-
Oryctolagus cuniculus
10
-
-
Pseudomonas putida

pH Stability

pH Stability pH Stability Maximum Comment Organism
4.5 7.3
-
Diutina rugosa
5.7 9.3
-
Pseudomonas putida
7 11.5
-
Oryctolagus cuniculus

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Oryctolagus cuniculus
NAD+
-
Pseudomonas putida
NAD+
-
Diutina rugosa