Protein Variants | Comment | Organism |
---|---|---|
additional information | xylitol production is increased by expression of codon-optimized Neurospora crassa xylose reductase gene under control of a constitutive glyceraldehyde-3-phosphate dehydrogenase promoter in a Candida tropicalis xylitol dehydrogenase gene (XYL2)-disrupted strain resulting in recombinant strain LNG2, overview | Candida tropicalis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
xylitol + NAD+ | Candida tropicalis | - |
D-xylose + NADH + H+ | - |
r | |
xylitol + NAD+ | Candida tropicalis ATCC 20336 | - |
D-xylose + NADH + H+ | - |
r | |
xylitol + NADP+ | Candida tropicalis | - |
D-xylose + NADPH + H+ | - |
r | |
xylitol + NADP+ | Candida tropicalis ATCC 20336 | - |
D-xylose + NADPH + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Candida tropicalis | - |
- |
- |
Candida tropicalis ATCC 20336 | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2.86 | - |
recombinant strain LNG2, pH 7.0, 25°C, xylose reduction with NADPH | Candida tropicalis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
xylitol + NAD+ | - |
Candida tropicalis | D-xylose + NADH + H+ | - |
r | |
xylitol + NAD+ | - |
Candida tropicalis ATCC 20336 | D-xylose + NADH + H+ | - |
r | |
xylitol + NADP+ | - |
Candida tropicalis | D-xylose + NADPH + H+ | - |
r | |
xylitol + NADP+ | - |
Candida tropicalis ATCC 20336 | D-xylose + NADPH + H+ | - |
r |
Synonyms | Comment | Organism |
---|---|---|
xylose reductase | - |
Candida tropicalis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Candida tropicalis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Candida tropicalis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Candida tropicalis | |
NADH | - |
Candida tropicalis | |
NADP+ | - |
Candida tropicalis | |
NADPH | - |
Candida tropicalis |
Organism | Comment | Expression |
---|---|---|
Candida tropicalis | formation of xylose reductase in the yeast Candida tropicalis is significantly repressed in cells grown on medium that contains glucose as carbon and energy source, because of the repressive effect of glucose | down |
General Information | Comment | Organism |
---|---|---|
metabolism | xylose reductase is the first enzyme in D-xylose metabolism, catalyzing the reduction of D-xylose to xylitol | Candida tropicalis |