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Literature summary for 1.1.1.304 extracted from

  • Giovannini, P.; Mantovani, M.; Grandini, A.; Medici, A.; Pedrini, P.
    New acetoin reductases from Bacillus stearothermophilus: meso- and 2R,3R-butanediol as fermentation products (2011), J. Mol. Catal. B, 69, 15-20.
No PubMed abstract available

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
diacetyl + NADH + H+ Geobacillus stearothermophilus
-
(S)-acetoin + NAD+
-
?
diacetyl + NADH + H+ Geobacillus stearothermophilus ATCC 2027
-
(S)-acetoin + NAD+
-
?
additional information Geobacillus stearothermophilus the enzyme shows an S-enantioselectivity in the reversible reduction of acetoin so it might be responsible of the meso-butenediol formation from R-acetoin. It acts on racemic acetoin and (S)-acetoin to form (2S,3S)-butane-2,3-diol, EC 1.1.1.76, but also on the (2R,3R)-butane-2,3-diol isomer in the reverse reaction, EC 1.1.1.4 ?
-
?
additional information Geobacillus stearothermophilus ATCC 2027 the enzyme shows an S-enantioselectivity in the reversible reduction of acetoin so it might be responsible of the meso-butenediol formation from R-acetoin. It acts on racemic acetoin and (S)-acetoin to form (2S,3S)-butane-2,3-diol, EC 1.1.1.76, but also on the (2R,3R)-butane-2,3-diol isomer in the reverse reaction, EC 1.1.1.4 ?
-
?

Organism

Organism UniProt Comment Textmining
Geobacillus stearothermophilus
-
-
-
Geobacillus stearothermophilus ATCC 2027
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme by adsorption on diethylaminoethyl–Sepharose and hydrophobic interaction chromatography Geobacillus stearothermophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
diacetyl + NADH + H+
-
Geobacillus stearothermophilus (S)-acetoin + NAD+
-
?
diacetyl + NADH + H+
-
Geobacillus stearothermophilus ATCC 2027 (S)-acetoin + NAD+
-
?
additional information the enzyme shows an S-enantioselectivity in the reversible reduction of acetoin so it might be responsible of the meso-butenediol formation from R-acetoin. It acts on racemic acetoin and (S)-acetoin to form (2S,3S)-butane-2,3-diol, EC 1.1.1.76, but also on the (2R,3R)-butane-2,3-diol isomer in the reverse reaction, EC 1.1.1.4 Geobacillus stearothermophilus ?
-
?
additional information the enzyme shows an S-enantioselectivity in the reversible reduction of acetoin so it might be responsible of the meso-butenediol formation from R-acetoin. It acts on racemic acetoin and (S)-acetoin to form (2S,3S)-butane-2,3-diol, EC 1.1.1.76, but also on the (2R,3R)-butane-2,3-diol isomer in the reverse reaction, EC 1.1.1.4 Geobacillus stearothermophilus ATCC 2027 ?
-
?

Synonyms

Synonyms Comment Organism
BSDR
-
Geobacillus stearothermophilus
S-stereospecific diacetyl reductase
-
Geobacillus stearothermophilus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
assay at Geobacillus stearothermophilus

Cofactor

Cofactor Comment Organism Structure
NADH dependent on Geobacillus stearothermophilus

General Information

General Information Comment Organism
metabolism the enzyme is involved in the butanediol cycle, overview Geobacillus stearothermophilus