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Literature summary for 1.1.1.304 extracted from
- Characterization of a stereospecific acetoin(diacetyl) reductase from Rhodococcus erythropolis WZ010 and its application for the synthesis of (2S,3S)-2,3-butanediol (2014), Appl. Microbiol. Biotechnol., 98, 641-650.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| DMSO | DMSO at a final concentration of 30% v/v added into the assay mixture, increases the activity up to 120% of the control enzyme activity | Rhodococcus erythropolis |  |
Application
| Application | Comment | Organism |
|---|---|---|
| synthesis | acetoin(diacetyl) reductase, i.e. 2,3-butanediol dehydrogenase, is one of the key enzymes in the microbial production of 2,3-butanediol, a platform with extensive industrial applications in the production of plastics, printing inks, perfumes, fumigants, spandex, moistening and softening agents, plasticizers, and pharmaceutical carrier | Rhodococcus erythropolis |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene adr, DNA and amino acid sequence determination and analysis, sequence comparison | Rhodococcus erythropolis |
| gene adr, DNA and amino acid sequence determination and analysis, sequence comparison, expression of His-tagged enzyme in Escherichia coli | Rhodococcus erythropolis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Al3+ | - |
Rhodococcus erythropolis | |
| Fe2+ | inhibits 91.6% at 2 mM | Rhodococcus erythropolis | |
| Zn2+ | inhibits 94.6% at 2 mM | Rhodococcus erythropolis | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | Michaelis-Menten-type kinetics | Rhodococcus erythropolis |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| K+ | activates by 201.6 to 265.6% at 2 mM | Rhodococcus erythropolis | |
| Mn2+ | activates by 201.6 to 265.6% at 2 mM | Rhodococcus erythropolis | |
| additional information | addition of EDTA or the cations at 1 mM, such as Na+, K+, Mn2+, Mg2+, and Ca2+, have no significant effect on the activity of ReADR | Rhodococcus erythropolis | |
| Na+ | activates by 201.6 to 265.6% at 2 mM | Rhodococcus erythropolis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 26864 | - |
2 * 26864, sequence calculation | Rhodococcus erythropolis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| diacetyl + NADH + H+ | Rhodococcus erythropolis | - |
(R)-acetoin + NAD+ | - |
r | |
| diacetyl + NADH + H+ | Rhodococcus erythropolis | - |
(S)-acetoin + NAD+ | - |
r | |
| diacetyl + NADH + H+ | Rhodococcus erythropolis PR4 | - |
(R)-acetoin + NAD+ | - |
r | |
| diacetyl + NADH + H+ | Rhodococcus erythropolis WZ010 | - |
(S)-acetoin + NAD+ | - |
r | |
| additional information | Rhodococcus erythropolis | Rhodococcus erythropolis WZ010 is capable of producing optically pure (2S,3S)-2,3-butanediol in alcoholic fermentation. | ? | - |
? | |
| additional information | Rhodococcus erythropolis WZ010 | Rhodococcus erythropolis WZ010 is capable of producing optically pure (2S,3S)-2,3-butanediol in alcoholic fermentation. | ? | - |
? | |
Organic Solvent Stability
| Organic Solvent | Comment | Organism |
|---|---|---|
| DMSO | the enzyme retains 53.6% of the initial activity after 4 h incubation with 30% v/v DMSO at 4°C | Rhodococcus erythropolis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rhodococcus erythropolis | - |
gene adr | - |
| Rhodococcus erythropolis | C0ZPN9 | gene adr | - |
| Rhodococcus erythropolis PR4 | C0ZPN9 | gene adr | - |
| Rhodococcus erythropolis WZ010 | - |
gene adr | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged enzyme from Escherichia coli by nickel affinity chromatography | Rhodococcus erythropolis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| diacetyl + NADH + H+ | - |
Rhodococcus erythropolis | (R)-acetoin + NAD+ | - |
r | |
| diacetyl + NADH + H+ | - |
Rhodococcus erythropolis PR4 | (R)-acetoin + NAD+ | - |
r | |
| diacetyl + NADH + H+ | - |
Rhodococcus erythropolis | (S)-acetoin + NAD+ | - |
r | |
| diacetyl + NADH + H+ | - |
Rhodococcus erythropolis WZ010 | (S)-acetoin + NAD+ | - |
r | |
| additional information | Rhodococcus erythropolis WZ010 is capable of producing optically pure (2S,3S)-2,3-butanediol in alcoholic fermentation. | Rhodococcus erythropolis | ? | - |
? | |
| additional information | the enzyme displays absolute stereospecificity in the reduction of diacetyl to (2S,3S)-2,3-butanediol via (S)-acetoin. The enzyme shows higher catalytic efficiency for (S)-1-phenylethanol oxidation than that for acetophenone reduction. ReADR-catalyzed asymmetric reduction of diacetyl is coupled with stereoselective oxidation of 1-phenylethanol, which simultaneously forms both (2S,3S)-2,3-butanediol and (R)-1-phenylethanol in great conversions and enantiomeric excess values.The enzyme accepts a broad range of substrates including aliphatic and aryl alcohols, aldehydes, and ketones | Rhodococcus erythropolis | ? | - |
? | |
| additional information | Rhodococcus erythropolis WZ010 is capable of producing optically pure (2S,3S)-2,3-butanediol in alcoholic fermentation. | Rhodococcus erythropolis WZ010 | ? | - |
? | |
| additional information | the enzyme displays absolute stereospecificity in the reduction of diacetyl to (2S,3S)-2,3-butanediol via (S)-acetoin. The enzyme shows higher catalytic efficiency for (S)-1-phenylethanol oxidation than that for acetophenone reduction. ReADR-catalyzed asymmetric reduction of diacetyl is coupled with stereoselective oxidation of 1-phenylethanol, which simultaneously forms both (2S,3S)-2,3-butanediol and (R)-1-phenylethanol in great conversions and enantiomeric excess values.The enzyme accepts a broad range of substrates including aliphatic and aryl alcohols, aldehydes, and ketones | Rhodococcus erythropolis WZ010 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | 2 * 26864, sequence calculation | Rhodococcus erythropolis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| acetoin(diacetyl) reductase | - |
Rhodococcus erythropolis |
| AdR | - |
Rhodococcus erythropolis |
| ReADR | - |
Rhodococcus erythropolis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
diacetyl reduction | Rhodococcus erythropolis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
diacetyl reduction | Rhodococcus erythropolis |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | - |
Rhodococcus erythropolis | |
| NADH | - |
Rhodococcus erythropolis | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | the enzyme belongs to the family of the short-chain dehydrogenase/reductases | Rhodococcus erythropolis |
| metabolism | acetoin(diacetyl) reductase, also known as 2,3-butanediol dehydrogenase, is one of the key enzymes in the microbial production of 2,3-butanediol | Rhodococcus erythropolis |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 8.519 | - |
NADH | pH 7.0, 30°C, recombinant enzyme | Rhodococcus erythropolis | |
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