Cloned (Comment) | Organism |
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gene rdh11, cloning of Rdh11/12-like 1-3 variants on the basis of template coding region of human rdh11 cDNA, DNA and amino acid determination and analysis, genetic structure, and quantitative RT-PCR expression analysis, sequence comparisons, recombinant expression of Rdh11/12-like 1 in HEK-293T cells resulting in no significant activity in conversion of T to A4 or E2 to E1 compared to the mock control | Anguilla japonica |
Organism | UniProt | Comment | Textmining |
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Anguilla japonica | - |
- |
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Source Tissue | Comment | Organism | Textmining |
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brain | - |
Anguilla japonica | - |
eye | - |
Anguilla japonica | - |
germ cell | - |
Anguilla japonica | - |
hypophysis | - |
Anguilla japonica | - |
Leydig cell | - |
Anguilla japonica | - |
additional information | quantitative RT-PCR expression analysis, expression pattern in different tissues. The pituitary and parts of brain and testis are the predominant sources of rdh11/12-like 1 transcript, while rdh11/12-like 2 mRNA is mostly detected in pituitary, parts of the brain, eye, and testis. Expression of rdh11/12-like 3, on the other hand, is ubiquitous across the tissues analyzed | Anguilla japonica | - |
Sertoli cell | - |
Anguilla japonica | - |
testis | Leydig cells, Sertoli cells, and germ cells: rdh11/12-like 1 and rdh11/12-like 3 transcripts are detected in all three fractions with no significant differences in expression level among the different fractions. In contrast, rdh11/12-like 2 mRNA is detected only in the germ cell fraction | Anguilla japonica | - |
Synonyms | Comment | Organism |
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More | see also EC 1.1.1.51 | Anguilla japonica |
RDH11 | - |
Anguilla japonica |
Rdh11/12-like 1 | - |
Anguilla japonica |
Rdh11/12-like 2 | - |
Anguilla japonica |
Rdh11/12-like 3 | - |
Anguilla japonica |
retinol dehydrogenases 11-like | - |
Anguilla japonica |
Cofactor | Comment | Organism | Structure |
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NADP+ | - |
Anguilla japonica | |
NADPH | - |
Anguilla japonica |
Organism | Comment | Expression |
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Anguilla japonica | recombinant equine gonadotropins (reFSH and reLH) and 11-oxotestosterone (11KT) have no effects on the expression of rdh11/12-like transcripts in the testis | additional information |
General Information | Comment | Organism |
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evolution | all three proteins (Rdh11/12-like 1-3) include conserved signatures of the SDR family, such as cofactor binding (TGXXXGXG), the catalytic mechanism (YXXXK), and the structural integrity (NVG or NAG) patterns. Japanese eel Rdh11/12-like 1 clusters with piscine Rdh11 and Rdh12, however the cluster is formed outside that of mammalian Rdh11 and Rdh12. In contrast, Rdh11/12-like 2 and Rdh11/12-like 3 form a clade with putative European eel Rdh11s and Rdh12s outside that of mammalian and piscine Rdh11/Rdh12 | Anguilla japonica |
physiological function | Japanese eel retinol dehydrogenases 11/12-like 1-3 (Rdh11/12-like 1-3) are 17-oxosteroid reductases (EC 1.1.1.51) involved in sex steroid synthesis. Catalysis of the conversion of A4 to T and E1 to E2 is performed by recombinant Rdh11/12-like 1 | Anguilla japonica |