Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Paracoccus denitrificans |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
27200 | - |
- |
Paracoccus denitrificans |
29000 | - |
4 * 29000, SDS-PAGE | Paracoccus denitrificans |
120000 | - |
gel filtration | Paracoccus denitrificans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R)-3-hydroxybutanoate + NAD+ | Paracoccus denitrificans | - |
acetoacetate + NADH + H+ | - |
? | |
(R)-3-hydroxybutanoate + NAD+ | Paracoccus denitrificans ATCC 17741 | - |
acetoacetate + NADH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Paracoccus denitrificans | - |
- |
- |
Paracoccus denitrificans ATCC 17741 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography, and TSK gel G3000SW gel filtration | Paracoccus denitrificans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R)-3-hydroxybutanoate + NAD+ | - |
Paracoccus denitrificans | acetoacetate + NADH + H+ | - |
? | |
(R)-3-hydroxybutanoate + NAD+ | - |
Paracoccus denitrificans ATCC 17741 | acetoacetate + NADH + H+ | - |
? | |
3-hydroxyvalerate + NAD+ | - |
Paracoccus denitrificans | 3-oxovalerate + NADH + H+ | - |
? | |
3-hydroxyvalerate + NAD+ | - |
Paracoccus denitrificans ATCC 17741 | 3-oxovalerate + NADH + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 4 * 29000, SDS-PAGE | Paracoccus denitrificans |
homotetramer | 4 * 27200, calculated from amino acid sequence | Paracoccus denitrificans |
Synonyms | Comment | Organism |
---|---|---|
HBD | - |
Paracoccus denitrificans |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Paracoccus denitrificans |