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Literature summary for 1.1.1.30 extracted from

  • Yeon, Y.J.; Park, H.Y.; Yoo, Y.J.
    Enzymatic reduction of levulinic acid by engineering the substrate specificity of 3-hydroxybutyrate dehydrogenase (2013), Biores. Technol., 134, 377-380.
    View publication on PubMed

Application

Application Comment Organism
synthesis the engineered enzyme mutant H144L/W187F is used for production of 4-hydroxyvaleric acid, a monomer of bio-polyester and a precursor of bio-fuels, from levulinic acid Alcaligenes faecalis

Cloned(Commentary)

Cloned (Comment) Organism
expression of C-terminally His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21 (DE3) Alcaligenes faecalis

Protein Variants

Protein Variants Comment Organism
H144L/W187F site-directed mutagenesis, the mutant shows activity with levulinnic acid, in contrast to the wild-type enzyme, and is engineered for production of 4-hydroxyvaleric acid, molecular docking simulation, overview Alcaligenes faecalis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(R)-3-hydroxybutanoate + NAD+ Alcaligenes faecalis
-
acetoacetate + NADH + H+
-
r

Organism

Organism UniProt Comment Textmining
Alcaligenes faecalis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant C-terminally His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21 (DE3) by nickel affinity chromatography Alcaligenes faecalis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(R)-3-hydroxybutanoate + NAD+
-
Alcaligenes faecalis acetoacetate + NADH + H+
-
r
additional information no activity of the wild-type enzyme with levulinic acid, enzyme mutant H144L/W187F is active with levulinic acid producing 4-hydroxyvaleric acid Alcaligenes faecalis ?
-
?

Synonyms

Synonyms Comment Organism
3HBDH
-
Alcaligenes faecalis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
reduction reaction Alcaligenes faecalis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
reduction reaction Alcaligenes faecalis

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Alcaligenes faecalis

General Information

General Information Comment Organism
additional information Gln94, His144, Lys152, and Gln196 form hydrogen bonds with carboxyl and/or ketone group of acetoacetate, Trp187, Trp257 form hydrophobic interactions with the carbon atoms of acetoacetate, and Ser142 and Tyr155 are directly related to the catalytic mechanism Alcaligenes faecalis