Cloned (Comment) | Organism |
---|---|
efficiently expressed in Escherichia coli cells harboring pHBDH11 | Pseudomonas fragi |
Crystallization (Comment) | Organism |
---|---|
hanging-drop vapor-diffusion method, ligand-free enzyme and enzyme-NAD+ complex, 2.0 A resolution | Pseudomonas fragi |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
cacodylate | competitive | Pseudomonas fragi | |
dimethyl malonate | - |
Pseudomonas fragi | |
DL-2-hydroxybutyrate | - |
Pseudomonas fragi | |
DL-lactate | - |
Pseudomonas fragi | |
malonate | - |
Pseudomonas fragi |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.13 | - |
NAD+ | - |
Pseudomonas fragi | |
1.2 | - |
(R)-3-hydroxybutanoate | - |
Pseudomonas fragi | |
7.4 | - |
NADP+ | - |
Pseudomonas fragi | |
388 | - |
L-threonine | - |
Pseudomonas fragi |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
27000 | - |
x * 27000, SDS-PAGE | Pseudomonas fragi |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas fragi | Q5KST5 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant | Pseudomonas fragi |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R)-3-hydroxybutanoate + NAD+ | - |
Pseudomonas fragi | acetoacetate + NADH | - |
? | |
(R)-3-hydroxybutanoate + NADP+ | activity with NADP+ is 2% of the activity with NAD+ | Pseudomonas fragi | acetoacetate + NADPH | - |
? | |
L-threonine + NAD+ | - |
Pseudomonas fragi | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 27000, SDS-PAGE | Pseudomonas fragi |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
reduction | Pseudomonas fragi |
8.5 | - |
dehydrogenation | Pseudomonas fragi |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Pseudomonas fragi | |
NADP+ | 2% of the activity with NAD+ | Pseudomonas fragi |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.81 | - |
DL-2-hydroxybutyrate | - |
Pseudomonas fragi | |
0.9 | - |
DL-lactate | - |
Pseudomonas fragi | |
3.8 | - |
malonate | - |
Pseudomonas fragi | |
5.6 | - |
cacodylate | - |
Pseudomonas fragi | |
80 | - |
dimethyl malonate | - |
Pseudomonas fragi |