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Literature summary for 1.1.1.30 extracted from

  • Takanashi, M.; Saito, T.
    Characterization of two 3-hydroxybutyrate dehydrogenases in poly(3-hydroxybutyrate)-degradable bacterium, Ralstonia pickettii T1 (2006), J. Biosci. Bioeng., 101, 501-507.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Ralstonia pickettii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.1
-
NAD+ 30°C Ralstonia pickettii
0.24
-
NADH 30°C Ralstonia pickettii
0.31
-
NAD+ 30°C Ralstonia pickettii
0.32
-
NADH 30°C Ralstonia pickettii
0.34
-
(R)-3-hydroxybutanoate 30°C Ralstonia pickettii
0.49
-
(R)-3-hydroxybutanoate 30°C Ralstonia pickettii
1.1
-
acetoacetate 30°C Ralstonia pickettii
1.3
-
acetoacetate 30°C Ralstonia pickettii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
31000
-
4 * 31000, SDS-PAGE Ralstonia pickettii
110000
-
gel filtration Ralstonia pickettii

Organism

Organism UniProt Comment Textmining
Ralstonia pickettii Q2PEN2
-
-
Ralstonia pickettii Q2PEN3
-
-
Ralstonia pickettii T1 Q2PEN2
-
-
Ralstonia pickettii T1 Q2PEN3
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Ralstonia pickettii

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
85
-
-
Ralstonia pickettii
180
-
-
Ralstonia pickettii
360
-
recombinant enzyme Ralstonia pickettii
450
-
recombinant enzyme Ralstonia pickettii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(R)-3-hydroxybutanoate + NAD+
-
Ralstonia pickettii acetoacetate + NADH + H+
-
r
(R)-3-hydroxybutanoate + NAD+
-
Ralstonia pickettii T1 acetoacetate + NADH + H+
-
r

Subunits

Subunits Comment Organism
tetramer 4 * 31000, SDS-PAGE Ralstonia pickettii

Synonyms

Synonyms Comment Organism
BDH1
-
Ralstonia pickettii
BDH2
-
Ralstonia pickettii
D-(-)-3-hydroxybutyrate dehydrogenase
-
Ralstonia pickettii

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
58
-
NADH 30°C Ralstonia pickettii
87
-
NAD+ 30°C Ralstonia pickettii
100
-
acetoacetate 30°C Ralstonia pickettii
110
-
acetoacetate 30°C Ralstonia pickettii
180
-
(R)-3-hydroxybutanoate 30°C Ralstonia pickettii
280
-
NADH 30°C Ralstonia pickettii
310
-
(R)-3-hydroxybutanoate 30°C Ralstonia pickettii
340
-
NAD+ 30°C Ralstonia pickettii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
reduction Ralstonia pickettii
6.5
-
reduction Ralstonia pickettii
7.5
-
oxidation Ralstonia pickettii
9
-
oxidation Ralstonia pickettii

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Ralstonia pickettii
NADH
-
Ralstonia pickettii