Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | 10 mM, 41% increase | Zoogloea ramigera I-16-M | |
dithiothreitol | 10 mM, 31% increase | Zoogloea ramigera I-16-M | |
EDTA | 0.5 mM, 21% increase | Zoogloea ramigera I-16-M | |
KCN | 0.1 mM, 37% increase | Zoogloea ramigera I-16-M | |
phosphatidylcholine | - |
Zoogloea ramigera I-16-M | |
reduced glutathione | 10 mM, 38% increase | Zoogloea ramigera I-16-M |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-Hydroxybutyrate | 10 mM, 57% inhibition | Zoogloea ramigera I-16-M | |
2-oxobutyrate | 10 mM, 29% inhibition | Zoogloea ramigera I-16-M | |
5,5'-dithiobis(2-nitrobenzoic acid) | 0.15 mM, 63% inhibition | Zoogloea ramigera I-16-M | |
acetoacetate | inhibition above 1 mM, 2 mM, 89% inhibition | Zoogloea ramigera I-16-M | |
acetoacetyl-CoA | 1 mM, 29% inhibition | Zoogloea ramigera I-16-M | |
acetyl-CoA | 1 mM, 82% inhibition | Zoogloea ramigera I-16-M | |
Butyrate | 10 mM, 20% inhibition | Zoogloea ramigera I-16-M | |
crotonate | 10 mM, 20% inhibition | Zoogloea ramigera I-16-M | |
CuSO4 | 1 mM, 81% inhibition | Zoogloea ramigera I-16-M | |
HgCl2 | 0.1 mM, 100% inhibition | Zoogloea ramigera I-16-M | |
L-3-hydroxybutyrate | 5 mM, 73% inhibition | Zoogloea ramigera I-16-M | |
malonate | 10 mM, 66% inhibition, oxidation | Zoogloea ramigera I-16-M | |
MnCl2 | 1 mM, 50% inhibition | Zoogloea ramigera I-16-M | |
NADH | 0.15 mM, 48% inhibition | Zoogloea ramigera I-16-M | |
p-chloromercuribenzoate | 0.1 mM, 100% inhibition | Zoogloea ramigera I-16-M | |
pyruvate | 4 mM, 5% inhibition | Zoogloea ramigera I-16-M | |
succinate | 10 mM, 12% inhibition | Zoogloea ramigera I-16-M |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.015 | - |
NADH | - |
Zoogloea ramigera I-16-M | |
0.057 | - |
NAD+ | - |
Zoogloea ramigera I-16-M | |
0.15 | - |
acetoacetate | - |
Zoogloea ramigera I-16-M | |
0.32 | - |
(R)-3-hydroxybutanoate | - |
Zoogloea ramigera I-16-M |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 10 mM 20% increase in activity | Zoogloea ramigera I-16-M | |
Mg2+ | 10 mM 41% increase in activity | Zoogloea ramigera I-16-M |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
28000 | - |
alpha4, 4 * 28000, SDS-PAGE | Zoogloea ramigera I-16-M |
112000 | - |
gel filtration | Zoogloea ramigera I-16-M |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R)-3-hydroxybutanoate + NAD+ | Zoogloea ramigera I-16-M | - |
acetoacetate + NADH | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Zoogloea ramigera I-16-M | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate, DEAE-Sepharose, 60°C, 10 min, hydroxylapatite, octyl-Sepharose, Sephadex G-200, DEAE-Sepharose | Zoogloea ramigera I-16-M |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
123 | - |
- |
Zoogloea ramigera I-16-M |
Storage Stability | Organism |
---|---|
-20°C, 1 mg enzyme/ml, 0.2 mM potassium phosphate pH 8.0, 10% glycerol, 10 mM 2-mercaptoethanol, 6 months, 0% inactivation, 0°C, 0.1 mg enzyme/ml, 1 day, 50% inactivation | Zoogloea ramigera I-16-M |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R)-3-hydroxybutanoate + NAD+ | - |
Zoogloea ramigera I-16-M | acetoacetate + NADH | - |
r | |
(R)-3-hydroxybutanoate + NAD+ | - |
Zoogloea ramigera I-16-M | acetoacetate + NADH + H+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
tetramer | alpha4, 4 * 28000, SDS-PAGE | Zoogloea ramigera I-16-M |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
oxidation of beta-hydroxybutyrate | Zoogloea ramigera I-16-M |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 7.5 | acetoacetate reduction | Zoogloea ramigera I-16-M |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Zoogloea ramigera I-16-M |