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Literature summary for 1.1.1.30 extracted from

  • Nakada T.; Fukui, T.; Saito, T.; Miki, K.; Oji, C.; Matsuda, S.; Ushijima, A.; Tomita, K.
    Purification and properties of D-beta-hydroxybutyrate dehydrogenase from Zoogloea ramiger I-16-M (1981), J. Biochem., 89, 625-635.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol 10 mM, 41% increase Zoogloea ramigera I-16-M
dithiothreitol 10 mM, 31% increase Zoogloea ramigera I-16-M
EDTA 0.5 mM, 21% increase Zoogloea ramigera I-16-M
KCN 0.1 mM, 37% increase Zoogloea ramigera I-16-M
phosphatidylcholine
-
Zoogloea ramigera I-16-M
reduced glutathione 10 mM, 38% increase Zoogloea ramigera I-16-M

Inhibitors

Inhibitors Comment Organism Structure
2-Hydroxybutyrate 10 mM, 57% inhibition Zoogloea ramigera I-16-M
2-oxobutyrate 10 mM, 29% inhibition Zoogloea ramigera I-16-M
5,5'-dithiobis(2-nitrobenzoic acid) 0.15 mM, 63% inhibition Zoogloea ramigera I-16-M
acetoacetate inhibition above 1 mM, 2 mM, 89% inhibition Zoogloea ramigera I-16-M
acetoacetyl-CoA 1 mM, 29% inhibition Zoogloea ramigera I-16-M
acetyl-CoA 1 mM, 82% inhibition Zoogloea ramigera I-16-M
Butyrate 10 mM, 20% inhibition Zoogloea ramigera I-16-M
crotonate 10 mM, 20% inhibition Zoogloea ramigera I-16-M
CuSO4 1 mM, 81% inhibition Zoogloea ramigera I-16-M
HgCl2 0.1 mM, 100% inhibition Zoogloea ramigera I-16-M
L-3-hydroxybutyrate 5 mM, 73% inhibition Zoogloea ramigera I-16-M
malonate 10 mM, 66% inhibition, oxidation Zoogloea ramigera I-16-M
MnCl2 1 mM, 50% inhibition Zoogloea ramigera I-16-M
NADH 0.15 mM, 48% inhibition Zoogloea ramigera I-16-M
p-chloromercuribenzoate 0.1 mM, 100% inhibition Zoogloea ramigera I-16-M
pyruvate 4 mM, 5% inhibition Zoogloea ramigera I-16-M
succinate 10 mM, 12% inhibition Zoogloea ramigera I-16-M

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.015
-
NADH
-
Zoogloea ramigera I-16-M
0.057
-
NAD+
-
Zoogloea ramigera I-16-M
0.15
-
acetoacetate
-
Zoogloea ramigera I-16-M
0.32
-
(R)-3-hydroxybutanoate
-
Zoogloea ramigera I-16-M

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 10 mM 20% increase in activity Zoogloea ramigera I-16-M
Mg2+ 10 mM 41% increase in activity Zoogloea ramigera I-16-M

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
28000
-
alpha4, 4 * 28000, SDS-PAGE Zoogloea ramigera I-16-M
112000
-
gel filtration Zoogloea ramigera I-16-M

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(R)-3-hydroxybutanoate + NAD+ Zoogloea ramigera I-16-M
-
acetoacetate + NADH
-
r

Organism

Organism UniProt Comment Textmining
Zoogloea ramigera I-16-M
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate, DEAE-Sepharose, 60°C, 10 min, hydroxylapatite, octyl-Sepharose, Sephadex G-200, DEAE-Sepharose Zoogloea ramigera I-16-M

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
123
-
-
Zoogloea ramigera I-16-M

Storage Stability

Storage Stability Organism
-20°C, 1 mg enzyme/ml, 0.2 mM potassium phosphate pH 8.0, 10% glycerol, 10 mM 2-mercaptoethanol, 6 months, 0% inactivation, 0°C, 0.1 mg enzyme/ml, 1 day, 50% inactivation Zoogloea ramigera I-16-M

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(R)-3-hydroxybutanoate + NAD+
-
Zoogloea ramigera I-16-M acetoacetate + NADH
-
r
(R)-3-hydroxybutanoate + NAD+
-
Zoogloea ramigera I-16-M acetoacetate + NADH + H+
-
r

Subunits

Subunits Comment Organism
tetramer alpha4, 4 * 28000, SDS-PAGE Zoogloea ramigera I-16-M

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
oxidation of beta-hydroxybutyrate Zoogloea ramigera I-16-M

pH Range

pH Minimum pH Maximum Comment Organism
5.5 7.5 acetoacetate reduction Zoogloea ramigera I-16-M

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Zoogloea ramigera I-16-M