Activating Compound | Comment | Organism | Structure |
---|---|---|---|
phosphatidylcholine | - |
Staphylococcus xylosus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000445 | - |
NAD+ | - |
Staphylococcus xylosus | |
0.00102 | - |
NADH | - |
Staphylococcus xylosus | |
0.00547 | - |
acetoacetate | - |
Staphylococcus xylosus | |
0.0391 | - |
(R)-3-hydroxybutanoate | - |
Staphylococcus xylosus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Staphylococcus xylosus | 5737 | - |
mitochondrion | - |
Staphylococcus xylosus | 5739 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 10 mM, more than 10fold increase in activity | Staphylococcus xylosus | |
Cd2+ | 10 mM, 2fold increase in activity | Staphylococcus xylosus | |
Cu2+ | 10 mM, 2fold increase in activity | Staphylococcus xylosus | |
Mg2+ | 10 mM, more than 10fold increase | Staphylococcus xylosus | |
Mn2+ | 10 mM, more than 10fold increase in activity | Staphylococcus xylosus | |
Zn2+ | 10 mM, 2fold increase in activity | Staphylococcus xylosus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
39000 | - |
alpha4, 4 * 39000, SDS-PAGE | Staphylococcus xylosus |
68000 | - |
nondenaturing PAGE | Staphylococcus xylosus |
140000 | - |
gel filtration | Staphylococcus xylosus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R)-3-hydroxybutanoate + NAD+ | Staphylococcus xylosus | - |
acetoacetate + NADH | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Staphylococcus xylosus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
protamine sulfate precipitation, Sephacryl S-400, S200, DEAE-Sepharose | Staphylococcus xylosus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.74 | - |
- |
Staphylococcus xylosus |
Storage Stability | Organism |
---|---|
-20°C, crude extract, partially purified enzyme, several weeks, 0% inactivation | Staphylococcus xylosus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R)-3-hydroxybutanoate + NAD+ | - |
Staphylococcus xylosus | acetoacetate + NADH | - |
r | |
(R)-3-hydroxybutanoate + NAD+ | - |
Staphylococcus xylosus | acetoacetate + NADH + H+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
tetramer | alpha4, 4 * 39000, SDS-PAGE | Staphylococcus xylosus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | 37 | - |
Staphylococcus xylosus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 49 | 80% activity at 25°C and at 49°C | Staphylococcus xylosus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
- |
45 | stable at 0°C and 25°C for 90 min, 18% activity after 90 min at 45°C, activity restored after 60 min at 37°C, loss of activity at 60°C | Staphylococcus xylosus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.8 | - |
acetoacetate reduction | Staphylococcus xylosus |
8.6 | - |
oxidation of (R)-3-hydroxybutanoate | Staphylococcus xylosus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.9 | 8.6 | acetoacetate reduction, 50% activity at pH 5.0, 66% at pH 8.6 | Staphylococcus xylosus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Staphylococcus xylosus |