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Literature summary for 1.1.1.30 extracted from
- Wild type and mutant human heart (R)-3-hydroxybutyrate dehydrogenase expressed in insect cells (1996), Biochemistry, 35, 8158-8165.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| phosphatidylcholine | - |
Homo sapiens |  |
| phosphatidylcholine | - |
Bos taurus | |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| mature form, expressed in Spodoptera frugiperda, SF9 cells | Homo sapiens |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| C242S | PCR derived cDNA clone | Homo sapiens |
| M92V | PCR derived cDNA clone | Homo sapiens |
| S24T | PCR derived cDNA clone | Homo sapiens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.53 | - |
NAD+ | enzyme expressed in SF9 cells | Homo sapiens | |
| 0.59 | - |
(R)-3-hydroxybutanoate | submitochondrial vesicles | Bos taurus | |
| 0.71 | - |
(R)-3-hydroxybutanoate | enzyme expressed in SF9 cells | Homo sapiens | |
| 0.8 | - |
NAD+ | enzyme expressed in SF9 cells, C242S mutant | Homo sapiens | |
| 0.95 | - |
NAD+ | submitochondrial vesicles | Bos taurus | |
| 10 | - |
(R)-3-hydroxybutanoate | enzyme expressed in SF9 cells, C242S mutant, C242 important for substrate binding | Homo sapiens | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | - |
Homo sapiens | 5737 | - |
| cytoplasm | - |
Bos taurus | 5737 | - |
| mitochondrion | located on the matrix face of the inner membrane | Homo sapiens | 5739 | - |
| mitochondrion | located on the matrix face of the inner membrane | Bos taurus | 5739 | - |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (R)-3-hydroxybutanoate + NAD+ | Homo sapiens | - |
acetoacetate + NADH | - |
r | |
| (R)-3-hydroxybutanoate + NAD+ | Bos taurus | - |
acetoacetate + NADH | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
- |
- |
| Homo sapiens | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| heart | - |
Homo sapiens | - |
| heart | - |
Bos taurus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.17 | - |
solubilized apo-3-hydroxybutyrate dehydrogenase, reconstituted with phosphatidylcholine/phosphatidylethanolamine/diphosphatidylglycerol, 5/4/1 | Bos taurus |
| 0.22 | - |
apo-3-hydroxybutyrate dehydrogenase, reconstituted with phosphatidylcholine/phosphatidylethanolamine/diphosphatidylglycerol, 5/4/1 | Homo sapiens |
| 0.5 | - |
enzyme expressed in SF9 cells, S24T mutatant | Homo sapiens |
| 0.52 | - |
submitochondrial vesicles, membrane bound enzyme | Bos taurus |
| 0.6 | - |
enzyme expressed in SF9 cells, M92V mutatant | Homo sapiens |
| 0.63 | - |
enzyme expressed in SF9 cells, membrane bound | Homo sapiens |
| 0.8 | - |
enzyme expressed in SF9 cells, C242S mutatant | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (R)-3-hydroxybutanoate + NAD+ | - |
Homo sapiens | acetoacetate + NADH | - |
r | |
| (R)-3-hydroxybutanoate + NAD+ | - |
Bos taurus | acetoacetate + NADH | - |
r | |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | - |
Homo sapiens | |
| NAD+ | - |
Bos taurus | |
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