Application | Comment | Organism |
---|---|---|
synthesis | contrary to wild-type MGA3 cells that secrete 0.4 g/l L-lysine and 59 g/l L-glutamate under optimised fed batch methanol fermentation, the hom-1 mutant M168-20 secretes 11 g/l L-lysine and 69 g/l of L-glutamate. Overproduction of pyruvate carboxylase and its mutant enzyme P455S in M168-20 has no positive effect on the volumetric L-lysine yield and the L-lysine yield on methanol, and causes significantly reduced volumetric L-glutamate yield and L-glutamate yield on methanol | Bacillus methanolicus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | heterologous expression in a hom-negative Escherichia coli mutant Gif 102, not able to grow on minimal medium unless added 1.5 mM of both L-threonine and L-methionine results in strains growing well on minimal agar plates without added threonine and methionine | Bacillus methanolicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus methanolicus | D8WXQ1 | isoform hom-1 | - |
Bacillus methanolicus | D8WXQ2 | isoform hom-2 | - |
Bacillus methanolicus MGA3 | D8WXQ1 | isoform hom-1 | - |
Bacillus methanolicus MGA3 | D8WXQ2 | isoform hom-2 | - |
Synonyms | Comment | Organism |
---|---|---|
hom-1 | - |
Bacillus methanolicus |
Organism | Comment | Expression |
---|---|---|
Bacillus methanolicus | strongly repressed by L-methionine | down |
Bacillus methanolicus | strongly repressed by L-threonine | down |
General Information | Comment | Organism |
---|---|---|
physiological function | contrary to wild-type MGA3 cells that secrete 0.4 g/l L-lysine and 59 g/l L-glutamate under optimised fed batch methanol fermentation, the hom-1 mutant M168-20 secretes 11 g/l L-lysine and 69 g/l of L-glutamate. Overproduction of pyruvate carboxylase and its mutant enzyme P455S in M168-20 has no positive effect on the volumetric L-lysine yield and the L-lysine yield on methanol, and causes significantly reduced volumetric L-glutamate yield and L-glutamate yield on methanol | Bacillus methanolicus |