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Literature summary for 1.1.1.3 extracted from

  • Paris, S.; Wessel, P.M.; Dumas, R.
    Overproduction, purification, and characterization of recombinant bifunctional threonine-sensitive aspartate kinase-homoserine dehydrogenase from Arabidopsis thaliana (2002), Protein Expr. Purif., 24, 105-110.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
L-threonine strong inhibition of both enzyme activities, aspartate dehydrogenase and aspartate kinase activity, by decreasing the affinity of the enzyme for substrate and cofactors, kinetic effects Arabidopsis thaliana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information kinetics Arabidopsis thaliana
0.166
-
NADP+ pH 8.0, 37°C, purified recombinant soluble enzyme Arabidopsis thaliana
5.2
-
L-homoserine pH 8.0, 37°C, purified recombinant soluble enzyme Arabidopsis thaliana
5.5
-
ATP pH 8.0, 37°C, purified recombinant soluble enzyme Arabidopsis thaliana
11.6
-
L-aspartate pH 8.0, 37°C, purified recombinant soluble enzyme Arabidopsis thaliana

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required for aspartate kinase activity Arabidopsis thaliana

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
320000
-
in presence of L-threonine, gel filtration Arabidopsis thaliana
470000
-
in absence of L-threonine, gel filtration Arabidopsis thaliana

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana O81852 slightly modified at base 60 c to t, bas 1242 t to g, and base 1403 t to c, the latter modification results in change of Leu468 to Ser; var. Columbia, bifunctional enzyme showing aspartate dehydrogenase and aspartate kinase activity
-

Reaction

Reaction Comment Organism Reaction ID
L-homoserine + NAD(P)+ = L-aspartate 4-semialdehyde + NAD(P)H + H+ bifunctional enzyme showing aspartate kinase, EC 2.7.2.4, and homoserine dehydrogenase activities Arabidopsis thaliana

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.4
-
purified recombinant soluble enzyme, forward reaction of aspartate kinase Arabidopsis thaliana
18.87
-
purified recombinant soluble enzyme, reverse reaction of homoserine dehydrogenase Arabidopsis thaliana

Storage Stability

Storage Stability Organism
-80°C, purified recombinant soluble enzyme, at 3 mg/ml, stable Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-homoserine + NADP+
-
Arabidopsis thaliana L-aspartate 4-semialdehyde + NADPH
-
r

Subunits

Subunits Comment Organism
hexamer in absence of L-threonine Arabidopsis thaliana
tetramer in presence of L-threonine Arabidopsis thaliana

Synonyms

Synonyms Comment Organism
AK-HSDH
-
Arabidopsis thaliana
aspartate kinase-homoserine dehydrogenase
-
Arabidopsis thaliana

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Arabidopsis thaliana

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Arabidopsis thaliana