Inhibitors | Comment | Organism | Structure |
---|---|---|---|
L-threonine | strong inhibition of both enzyme activities, aspartate dehydrogenase and aspartate kinase activity, by decreasing the affinity of the enzyme for substrate and cofactors, kinetic effects | Arabidopsis thaliana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics | Arabidopsis thaliana | |
0.166 | - |
NADP+ | pH 8.0, 37°C, purified recombinant soluble enzyme | Arabidopsis thaliana | |
5.2 | - |
L-homoserine | pH 8.0, 37°C, purified recombinant soluble enzyme | Arabidopsis thaliana | |
5.5 | - |
ATP | pH 8.0, 37°C, purified recombinant soluble enzyme | Arabidopsis thaliana | |
11.6 | - |
L-aspartate | pH 8.0, 37°C, purified recombinant soluble enzyme | Arabidopsis thaliana |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required for aspartate kinase activity | Arabidopsis thaliana |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
320000 | - |
in presence of L-threonine, gel filtration | Arabidopsis thaliana |
470000 | - |
in absence of L-threonine, gel filtration | Arabidopsis thaliana |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | O81852 | slightly modified at base 60 c to t, bas 1242 t to g, and base 1403 t to c, the latter modification results in change of Leu468 to Ser; var. Columbia, bifunctional enzyme showing aspartate dehydrogenase and aspartate kinase activity | - |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
L-homoserine + NAD(P)+ = L-aspartate 4-semialdehyde + NAD(P)H + H+ | bifunctional enzyme showing aspartate kinase, EC 2.7.2.4, and homoserine dehydrogenase activities | Arabidopsis thaliana |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
5.4 | - |
purified recombinant soluble enzyme, forward reaction of aspartate kinase | Arabidopsis thaliana |
18.87 | - |
purified recombinant soluble enzyme, reverse reaction of homoserine dehydrogenase | Arabidopsis thaliana |
Storage Stability | Organism |
---|---|
-80°C, purified recombinant soluble enzyme, at 3 mg/ml, stable | Arabidopsis thaliana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-homoserine + NADP+ | - |
Arabidopsis thaliana | L-aspartate 4-semialdehyde + NADPH | - |
r |
Subunits | Comment | Organism |
---|---|---|
hexamer | in absence of L-threonine | Arabidopsis thaliana |
tetramer | in presence of L-threonine | Arabidopsis thaliana |
Synonyms | Comment | Organism |
---|---|---|
AK-HSDH | - |
Arabidopsis thaliana |
aspartate kinase-homoserine dehydrogenase | - |
Arabidopsis thaliana |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Arabidopsis thaliana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Arabidopsis thaliana |