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Literature summary for 1.1.1.299 extracted from

  • Oikawa, T.; Yamamoto, N.; Shimoke, K.; Uesato, S.; Ikeuchi, T.; Fujioka, T.
    Purification, characterization, and overexpression of psychrophilic and thermolabile malate dehydrogenase of a novel antarctic psychrotolerant, Flavobacterium frigidimaris KUC-1 (2005), Biosci. Biotechnol. Biochem., 69, 2146-2154.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
CuCl2 1 mM, 10% residual activity Flavobacterium frigidimaris
HgCl2 1 mM, no residual activity Flavobacterium frigidimaris
iodoacetate 1 mM, 16.4% residual activity Flavobacterium frigidimaris
N-ethylmaleimide 1 mM, no residual activity Flavobacterium frigidimaris
ZnCl2 1 mM, 27% residual activity Flavobacterium frigidimaris

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.028
-
NAD+ 10°C Flavobacterium frigidimaris
0.029
-
NAD+ 20°C Flavobacterium frigidimaris
0.03
-
NAD+ 30°C Flavobacterium frigidimaris
0.038
-
NAD+ 40°C Flavobacterium frigidimaris
0.269
-
L-malate 20°C Flavobacterium frigidimaris
0.288
-
L-malate 30°C Flavobacterium frigidimaris
0.543
-
L-malate 10°C Flavobacterium frigidimaris
0.74
-
L-malate 40°C Flavobacterium frigidimaris

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
32000
-
4 * 32000, SDS-PAGE Flavobacterium frigidimaris
123000
-
gel filtration Flavobacterium frigidimaris

Organism

Organism UniProt Comment Textmining
Flavobacterium frigidimaris
-
-
-
Flavobacterium frigidimaris KUC-1
-
-
-

Reaction

Reaction Comment Organism Reaction ID
(S)-malate + NAD(P)+ = oxaloacetate + NAD(P)H + H+ ordered bi-bi mechanism Flavobacterium frigidimaris

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
176
-
30°C Flavobacterium frigidimaris

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-malate + NAD+
-
Flavobacterium frigidimaris oxaloacetate + NADH + H+
-
?
(S)-malate + NAD+
-
Flavobacterium frigidimaris KUC-1 oxaloacetate + NADH + H+
-
?
(S)-malate + NADP+
-
Flavobacterium frigidimaris oxaloacetate + NADPH + H+
-
?
(S)-malate + NADP+
-
Flavobacterium frigidimaris KUC-1 oxaloacetate + NADPH + H+
-
?
additional information enzyme shows pro-R stereospecificity for hydrogen transfer at the C4 position of the nicotinamide moiety of the coenzyme. No substrates are: D-malate, malonate, L-glutamate, L-aspartate, D,L-2-hydroxybutanoate, D,L-3-hydroxybutanoate, citrate, maleate, succinate, L-tartrate, L-threonine, L-serine, L-hydroxymalonate, D-glutamate, 2-oxocaproate, 2-oxoisovalerate, glyoxylate, 2-oxoglutarate, 2-oxobutanoate Flavobacterium frigidimaris ?
-
?
additional information enzyme shows pro-R stereospecificity for hydrogen transfer at the C4 position of the nicotinamide moiety of the coenzyme. No substrates are: D-malate, malonate, L-glutamate, L-aspartate, D,L-2-hydroxybutanoate, D,L-3-hydroxybutanoate, citrate, maleate, succinate, L-tartrate, L-threonine, L-serine, L-hydroxymalonate, D-glutamate, 2-oxocaproate, 2-oxoisovalerate, glyoxylate, 2-oxoglutarate, 2-oxobutanoate Flavobacterium frigidimaris KUC-1 ?
-
?
oxaloacetate + NADH + H+
-
Flavobacterium frigidimaris L-malate + NAD+
-
?

Subunits

Subunits Comment Organism
tetramer 4 * 32000, SDS-PAGE Flavobacterium frigidimaris

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
highest initial velocity Flavobacterium frigidimaris

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
5 60
-
Flavobacterium frigidimaris

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
35
-
half-life 26 min Flavobacterium frigidimaris
40
-
half-life 3 min Flavobacterium frigidimaris

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
41
-
NAD+ 10°C Flavobacterium frigidimaris
41.3
-
L-malate 10°C Flavobacterium frigidimaris
48.4
-
L-malate 20°C Flavobacterium frigidimaris
48.6
-
NAD+ 20°C Flavobacterium frigidimaris
83.2
-
L-malate 30°C Flavobacterium frigidimaris
83.4
-
NAD+ 30°C Flavobacterium frigidimaris
138
-
L-malate 40°C Flavobacterium frigidimaris
139
-
NAD+ 40°C Flavobacterium frigidimaris

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
reduction of oxaloacetate Flavobacterium frigidimaris
10.5
-
oxidation of malate Flavobacterium frigidimaris

pH Range

pH Minimum pH Maximum Comment Organism
4 10 reduction of oxaloacetate Flavobacterium frigidimaris
6.5 12 oxidation of malate Flavobacterium frigidimaris

pH Stability

pH Stability pH Stability Maximum Comment Organism
4 10.5
-
Flavobacterium frigidimaris

Cofactor

Cofactor Comment Organism Structure
additional information no cofactor: NADPH Flavobacterium frigidimaris
NAD+ relative activity of NAD+ to NADP+ is 43.9% Flavobacterium frigidimaris
NADH
-
Flavobacterium frigidimaris
NADP+ relative activity of NAD+ to NADP+ is 43.9% Flavobacterium frigidimaris

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
289
-
L-malate 30°C Flavobacterium frigidimaris
2790
-
NAD+ 30°C Flavobacterium frigidimaris