Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-hydroxypropanoate + NADP+ | Chloroflexus aurantiacus | - |
malonate-semialdehyde + NADPH + H+ | - |
r | |
malonate-semialdehyde + NADPH + H+ | Chloroflexus aurantiacus | - |
3-hydroxypropionate + NADP+ | - |
r | |
additional information | Chloroflexus aurantiacus | enzyme is part of an autotrophic CO2 fixation pathway in which acetyl-CoA is carboxylated and reductively converted via 3-hydroxypropionate to propionyl-CoA. Propionyl-CoA is carboxylated and converted via succinyl-CoA and CoA transfer to malyl-CoA. Malyl-CoA is cleaved to acetyl-CoA and glyoxylate. Thereby, the first CO, acceptor molecule acetyl-CoA is regenerated, completing the cycle and the net CO, fixation product glyoxylate is released | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Chloroflexus aurantiacus | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.002 | - |
substrate 3-hydroxypropionate, pH 9.0, 45°C | Chloroflexus aurantiacus |
0.04 | - |
substrate malonate-semialdehyde, pH 9.0, 45°C | Chloroflexus aurantiacus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-hydroxypropanoate + NADP+ | - |
Chloroflexus aurantiacus | malonate-semialdehyde + NADPH + H+ | - |
r | |
malonate-semialdehyde + NADPH + H+ | - |
Chloroflexus aurantiacus | 3-hydroxypropionate + NADP+ | - |
r | |
additional information | enzyme is part of an autotrophic CO2 fixation pathway in which acetyl-CoA is carboxylated and reductively converted via 3-hydroxypropionate to propionyl-CoA. Propionyl-CoA is carboxylated and converted via succinyl-CoA and CoA transfer to malyl-CoA. Malyl-CoA is cleaved to acetyl-CoA and glyoxylate. Thereby, the first CO, acceptor molecule acetyl-CoA is regenerated, completing the cycle and the net CO, fixation product glyoxylate is released | Chloroflexus aurantiacus | ? | - |
? |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | no cofactor: NAD+ | Chloroflexus aurantiacus | |
NADP+ | - |
Chloroflexus aurantiacus | |
NADPH | - |
Chloroflexus aurantiacus |