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Literature summary for 1.1.1.292 extracted from

  • Sakuma, M.; Kubota, S.
    Mouse AKR1E1 is an ortholog of pig liver NADPH dependent 1,5-anhydro-D-fructose reductase (2008), Biosci. Biotechnol. Biochem., 72, 872-876.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
AKR1E1 gene, cloning from liver RNA, DNA and amino acid sequence determination, analysis, and comparison, expression in Escherichia coli strain BL21 (DE3) Mus musculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.02
-
1,5-Anhydro-D-fructose pH 7.0, 37°C, recombinant enzyme Mus musculus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1,5-anhydro-D-mannitol + NADP+ Mus musculus
-
1,5-anhydro-D-fructose + NADPH
-
r
1,5-anhydro-D-mannitol + NADP+ Mus musculus C57/BL6J
-
1,5-anhydro-D-fructose + NADPH
-
r

Organism

Organism UniProt Comment Textmining
Mus musculus Q9DCT1 C57BL/6J mice
-
Mus musculus C57/BL6J Q9DCT1 C57BL/6J mice
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme 17.6fold from Escherichia coli strain BL21 (DE3) by anion exchange and adsorption chromatography Mus musculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,5-anhydro-D-mannitol + NADP+
-
Mus musculus 1,5-anhydro-D-fructose + NADPH
-
r
1,5-anhydro-D-mannitol + NADP+ 1,5-anhydro-D-fructose is the preferred substrate for the reductase reaction Mus musculus 1,5-anhydro-D-fructose + NADPH
-
r
1,5-anhydro-D-mannitol + NADP+
-
Mus musculus C57/BL6J 1,5-anhydro-D-fructose + NADPH
-
r
1,5-anhydro-D-mannitol + NADP+ 1,5-anhydro-D-fructose is the preferred substrate for the reductase reaction Mus musculus C57/BL6J 1,5-anhydro-D-fructose + NADPH
-
r
9,10-phenanthrenequinone + NADPH
-
Mus musculus ? + NADP+
-
?
9,10-phenanthrenequinone + NADPH
-
Mus musculus C57/BL6J ? + NADP+
-
?
butane-2,3-dione + NADPH
-
Mus musculus acetoin + NADP+
-
?
butane-2,3-dione + NADPH
-
Mus musculus C57/BL6J acetoin + NADP+
-
?
additional information D-glucose, D-fructose, glucuronic acid, DL-glyceraldehyde, formaldehyde, and acetone are poor substrates, substrate specificity of the recombinant enzyme, overview Mus musculus ?
-
?
additional information D-glucose, D-fructose, glucuronic acid, DL-glyceraldehyde, formaldehyde, and acetone are poor substrates, substrate specificity of the recombinant enzyme, overview Mus musculus C57/BL6J ?
-
?

Synonyms

Synonyms Comment Organism
AF reductase
-
Mus musculus
AKR1E1
-
Mus musculus
More the enzyme is a member of the aldo-keto reductase family 1 Mus musculus
NADPH dependent 1,5-anhydro-D-fructose reductase
-
Mus musculus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Mus musculus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
9.3
-
1,5-Anhydro-D-fructose pH 7.0, 37°C, recombinant enzyme Mus musculus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
assay at, reverse reaction Mus musculus