Cloned (Comment) | Organism |
---|---|
AKR1E1 gene, cloning from liver RNA, DNA and amino acid sequence determination, analysis, and comparison, expression in Escherichia coli strain BL21 (DE3) | Mus musculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.02 | - |
1,5-Anhydro-D-fructose | pH 7.0, 37°C, recombinant enzyme | Mus musculus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,5-anhydro-D-mannitol + NADP+ | Mus musculus | - |
1,5-anhydro-D-fructose + NADPH | - |
r | |
1,5-anhydro-D-mannitol + NADP+ | Mus musculus C57/BL6J | - |
1,5-anhydro-D-fructose + NADPH | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | Q9DCT1 | C57BL/6J mice | - |
Mus musculus C57/BL6J | Q9DCT1 | C57BL/6J mice | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme 17.6fold from Escherichia coli strain BL21 (DE3) by anion exchange and adsorption chromatography | Mus musculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,5-anhydro-D-mannitol + NADP+ | - |
Mus musculus | 1,5-anhydro-D-fructose + NADPH | - |
r | |
1,5-anhydro-D-mannitol + NADP+ | 1,5-anhydro-D-fructose is the preferred substrate for the reductase reaction | Mus musculus | 1,5-anhydro-D-fructose + NADPH | - |
r | |
1,5-anhydro-D-mannitol + NADP+ | - |
Mus musculus C57/BL6J | 1,5-anhydro-D-fructose + NADPH | - |
r | |
1,5-anhydro-D-mannitol + NADP+ | 1,5-anhydro-D-fructose is the preferred substrate for the reductase reaction | Mus musculus C57/BL6J | 1,5-anhydro-D-fructose + NADPH | - |
r | |
9,10-phenanthrenequinone + NADPH | - |
Mus musculus | ? + NADP+ | - |
? | |
9,10-phenanthrenequinone + NADPH | - |
Mus musculus C57/BL6J | ? + NADP+ | - |
? | |
butane-2,3-dione + NADPH | - |
Mus musculus | acetoin + NADP+ | - |
? | |
butane-2,3-dione + NADPH | - |
Mus musculus C57/BL6J | acetoin + NADP+ | - |
? | |
additional information | D-glucose, D-fructose, glucuronic acid, DL-glyceraldehyde, formaldehyde, and acetone are poor substrates, substrate specificity of the recombinant enzyme, overview | Mus musculus | ? | - |
? | |
additional information | D-glucose, D-fructose, glucuronic acid, DL-glyceraldehyde, formaldehyde, and acetone are poor substrates, substrate specificity of the recombinant enzyme, overview | Mus musculus C57/BL6J | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AF reductase | - |
Mus musculus |
AKR1E1 | - |
Mus musculus |
More | the enzyme is a member of the aldo-keto reductase family 1 | Mus musculus |
NADPH dependent 1,5-anhydro-D-fructose reductase | - |
Mus musculus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Mus musculus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
9.3 | - |
1,5-Anhydro-D-fructose | pH 7.0, 37°C, recombinant enzyme | Mus musculus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at, reverse reaction | Mus musculus |