Cloned (Comment) | Organism |
---|---|
overexpression in Escherichia coli | Ensifer adhaerens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.2 | - |
NADPH | pH 6.5, 30°C | Ensifer adhaerens | |
8.4 | - |
1,5-Anhydro-D-fructose | pH 6.5, 30°C | Ensifer adhaerens | |
11 | - |
D-glucosone | pH 6.5, 30°C | Ensifer adhaerens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35100 | - |
1 * 35100, MALDI-TOF-MS | Ensifer adhaerens |
38200 | - |
gel filtration | Ensifer adhaerens |
40000 | - |
1 * 40000, SDS-PAGE | Ensifer adhaerens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Ensifer adhaerens | Q2I8V6 | i.e. Ensifer adhaerens | - |
Ensifer adhaerens S-30.7.5 | Q2I8V6 | i.e. Ensifer adhaerens | - |
Purification (Comment) | Organism |
---|---|
native and recombinant enzyme | Ensifer adhaerens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
288.9 | - |
native enzyme | Ensifer adhaerens |
484 | - |
recombinant enzyme expressed in Escherichia coli | Ensifer adhaerens |
Storage Stability | Organism |
---|---|
-20°C or 0°C, 50 days, 50% loss of activity | Ensifer adhaerens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,5-anhydro-D-fructose + NADPH + H+ | - |
Ensifer adhaerens | 1,5-anhydro-D-mannitol + NADP+ | - |
r | |
1,5-anhydro-D-fructose + NADPH + H+ | - |
Ensifer adhaerens S-30.7.5 | 1,5-anhydro-D-mannitol + NADP+ | - |
r | |
1,5-anhydro-D-glucitol + NADP+ | 23% of the activity with 1,5-anhydro-D-mannitol | Ensifer adhaerens | ? | - |
? | |
1,5-anhydro-D-glucitol + NADP+ | 23% of the activity with 1,5-anhydro-D-mannitol | Ensifer adhaerens S-30.7.5 | ? | - |
? | |
6-deoxy-D-glucosone + NADPH + H+ | 22% of the activity with 1,5-anhydro-D-fructose | Ensifer adhaerens | 6-deoxy-D-mannose + NADP+ | - |
? | |
6-deoxy-D-glucosone + NADPH + H+ | 22% of the activity with 1,5-anhydro-D-fructose | Ensifer adhaerens S-30.7.5 | 6-deoxy-D-mannose + NADP+ | - |
? | |
D-allosone + NADPH + H+ | 10% of the activity with 1,5-anhydro-D-fructose | Ensifer adhaerens | D-altrose + NADP+ | - |
? | |
D-allosone + NADPH + H+ | 10% of the activity with 1,5-anhydro-D-fructose | Ensifer adhaerens S-30.7.5 | D-altrose + NADP+ | - |
? | |
D-glucosone + NADPH + H+ | 22% of the activity with 1,5-anhydro-D-fructose | Ensifer adhaerens | D-mannose + NADP+ | - |
? | |
D-glucosone + NADPH + H+ | 22% of the activity with 1,5-anhydro-D-fructose | Ensifer adhaerens S-30.7.5 | D-mannose + NADP+ | - |
? | |
D-xylosone + NADPH + H+ | 17% of the activity with 1,5-anhydro-D-fructose | Ensifer adhaerens | D-lyxose + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 40000, SDS-PAGE | Ensifer adhaerens |
monomer | 1 * 35100, MALDI-TOF-MS | Ensifer adhaerens |
Synonyms | Comment | Organism |
---|---|---|
1,5-anhydro-D-fructose reductase (ambiguous) | - |
Ensifer adhaerens |
AFR | - |
Ensifer adhaerens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
63.2 | - |
D-glucosone | pH 6.5, 30°C | Ensifer adhaerens | |
286.7 | - |
1,5-Anhydro-D-fructose | pH 6.5, 30°C | Ensifer adhaerens | |
1300 | - |
1,5-Anhydro-D-fructose | pH 6.5, 30°C | Ensifer adhaerens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
- |
Ensifer adhaerens |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.2 | 8.8 | about 50% of maximal activity at pH 5.5 and at pH 8.8 | Ensifer adhaerens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Ensifer adhaerens | |
NADPH | inactive towards NADH | Ensifer adhaerens |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Ensifer adhaerens | isoelectric focusing | - |
4.3 |