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Literature summary for 1.1.1.290 extracted from

  • Ha, J.Y.; Lee, J.H.; Kim, K.H.; Kim, d.o..J.; Lee, H.H.; Kim, H.K.; Yoon, H.J.; Suh, S.W.
    Crystal structure of D-erythronate-4-phosphate dehydrogenase complexed with NAD (2007), J. Mol. Biol., 366, 1294-1304.
    View publication on PubMed

Crystallization (Commentary)

Crystallization (Comment) Organism
purified recombinant C-terminally His8-tagged and selenomethionine-labeled enzyme, subunit A is bound with NAD+ and a phosphate ion, while subunit B, with a more open active site cleft, is bound with NAD+ and L(+)-tartrate, cryoprotection by 30% glycerol, X-ray diffraction structure determination and analysis at 2.3 A resolution, asymmetric subunit conformation and strucure comparison, overview Pseudomonas aeruginosa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4-phospho-D-erythronate + NAD+ Pseudomonas aeruginosa the enzyme is involved in biosynthesis of pyridoxal-5'-phosphate (3R)-3-hydroxy-2-oxo-4-phosphooxybutanoate + NADH
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Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa Q9I3W9 gene pdxB
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Reaction

Reaction Comment Organism Reaction ID
4-phospho-D-erythronate + NAD+ = (3R)-3-hydroxy-2-oxo-4-phosphooxybutanoate + NADH + H+ substrate recognition and catalytic mechanism, PdxB contains the conserved His254/Glu237/Arg208 triad Pseudomonas aeruginosa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-phospho-D-erythronate + NAD+
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Pseudomonas aeruginosa (3R)-3-hydroxy-2-oxo-4-phosphooxybutanoate + NADH
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4-phospho-D-erythronate + NAD+ the enzyme is involved in biosynthesis of pyridoxal-5'-phosphate Pseudomonas aeruginosa (3R)-3-hydroxy-2-oxo-4-phosphooxybutanoate + NADH
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additional information active site and ligand binding structure, overview Pseudomonas aeruginosa ?
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Subunits

Subunits Comment Organism
dimer homodimer, each subunit consists of three structural domains: the lid domain, the nucleotide-binding domain, and the C-terminal dimerization domain with a unique fold responsible for the dimerization, crystal structure analysis, overview Pseudomonas aeruginosa

Synonyms

Synonyms Comment Organism
D-erythronate-4-phosphate dehydrogenase
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Pseudomonas aeruginosa
More PdxB is a member of the D-isomer specific 2-hydroxyacid dehydrogenase superfamily Pseudomonas aeruginosa
PDXB
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Pseudomonas aeruginosa

Cofactor

Cofactor Comment Organism Structure
NAD+ NAD+ is bound to the C-terminal side of the beta-sheet of the nucleotide-binding domain in both subunits of PdxB, binding structure, overview Pseudomonas aeruginosa