Inhibitors | Comment | Organism | Structure |
---|---|---|---|
AgNO3 | - |
Hyphomicrobium methylovorum | |
CuSO4 | slight | Hyphomicrobium methylovorum | |
Dihydroxyfumarate | - |
Hyphomicrobium methylovorum | |
HgCl2 | - |
Hyphomicrobium methylovorum | |
additional information | not inhibitory: citrate, NADP+, EDTA, 1,10-phenanthroline, iodoacetate, iodoacetamide, N-ethylmaleimide, hydroxylamine, semicarbazide, NaN3, NaF, KCN, L-ascorbate, dithiothreitol, pyridoxal 5'-phosphate | Hyphomicrobium methylovorum | |
p-chloromercuribenzoate | - |
Hyphomicrobium methylovorum | |
phenylhydrazine | - |
Hyphomicrobium methylovorum | |
ZnCl2 | slight | Hyphomicrobium methylovorum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.055 | - |
NADH | - |
Hyphomicrobium methylovorum | |
0.175 | - |
Hydroxypyruvate | - |
Hyphomicrobium methylovorum | |
10.8 | - |
glyoxylate | - |
Hyphomicrobium methylovorum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
38000 | - |
2 * 38000, SDS-PAGE | Hyphomicrobium methylovorum |
69000 | 70000 | gel filtration | Hyphomicrobium methylovorum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
hydroxypyruvate + NADH | Hyphomicrobium methylovorum | - |
D-glycerate + NAD+ | - |
ir |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Hyphomicrobium methylovorum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Hyphomicrobium methylovorum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1086 | - |
- |
Hyphomicrobium methylovorum |
Storage Stability | Organism |
---|---|
-20°C, 10 mM potassium phosphate buffer, pH 7.0, 45% v/v glycerol, 1.0 M DTT, 18 months | Hyphomicrobium methylovorum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glyoxylate + NADH | - |
Hyphomicrobium methylovorum | glycolate + NAD+ | - |
? | |
hydroxypyruvate + NADH | - |
Hyphomicrobium methylovorum | D-glycerate + NAD+ | - |
ir |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 38000, SDS-PAGE | Hyphomicrobium methylovorum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
up to | Hyphomicrobium methylovorum |
30 | - |
30 min, 13.3% loss of activity | Hyphomicrobium methylovorum |
35 | - |
30 min, 30.8% loss of activity | Hyphomicrobium methylovorum |
40 | - |
30 min, 100% loss of activity | Hyphomicrobium methylovorum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.8 | - |
- |
Hyphomicrobium methylovorum |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | 9 | - |
Hyphomicrobium methylovorum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | specific for, not replaceable by NADPH | Hyphomicrobium methylovorum |