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Literature summary for 1.1.1.289 extracted from

  • Adachi, O.; Ano, Y.; Moonmangmee, D.; Shinagawa, E.; Toyama, H.; Theeragool, G.; Lotong, N.; Matsushita, K.
    Crystallization and properties of NADPH-dependent L-sorbose reductase from Gluconobacter melanogenus IFO 3294 (1999), Biosci. Biotechnol. Biochem., 63, 2137-2143.
    View publication on PubMed

Crystallization (Commentary)

Crystallization (Comment) Organism
-
Gluconobacter oxydans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.032
-
NADPH pH 6.0, 25°C Gluconobacter oxydans
35
-
L-sorbose pH 6.0, 25°C Gluconobacter oxydans

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Gluconobacter oxydans 5829
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30000
-
2 * 30000, SDS-PAGE Gluconobacter oxydans
60000
-
gel filtration Gluconobacter oxydans

Organism

Organism UniProt Comment Textmining
Gluconobacter oxydans
-
-
-
Gluconobacter oxydans IFO 3294
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Gluconobacter oxydans

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
77
-
pH 6.0, 25°C, substrate L-sorbose Gluconobacter oxydans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-sorbitol + NADP+
-
Gluconobacter oxydans L-sorbose + NADPH + H+
-
r
D-sorbitol + NADP+
-
Gluconobacter oxydans IFO 3294 L-sorbose + NADPH + H+
-
r
L-sorbose + NADPH + H+
-
Gluconobacter oxydans D-sorbitol + NADP+
-
r
L-sorbose + NADPH + H+
-
Gluconobacter oxydans IFO 3294 D-sorbitol + NADP+
-
r
additional information highly specific for D-sorbitol and L-sorbose. Reaction rate in L-sorbose reduction highly predominates over L-sorbitol oxidation over a wide pH range Gluconobacter oxydans ?
-
?
additional information highly specific for D-sorbitol and L-sorbose. Reaction rate in L-sorbose reduction highly predominates over L-sorbitol oxidation over a wide pH range Gluconobacter oxydans IFO 3294 ?
-
?

Subunits

Subunits Comment Organism
dimer 2 * 30000, SDS-PAGE Gluconobacter oxydans

Synonyms

Synonyms Comment Organism
L-sorbose reductase
-
Gluconobacter oxydans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
reduction of L-sorbose Gluconobacter oxydans
8
-
oxidation of D-sorbitol Gluconobacter oxydans

pH Range

pH Minimum pH Maximum Comment Organism
5 7 reduction of L-sorbose Gluconobacter oxydans
7 9 oxidation of D-sorbitol Gluconobacter oxydans

Cofactor

Cofactor Comment Organism Structure
additional information no cofactor: NAD+, NADH Gluconobacter oxydans
NADP+
-
Gluconobacter oxydans
NADPH
-
Gluconobacter oxydans