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Literature summary for 1.1.1.289 extracted from

  • Sugisawa, T.; Hoshino, T.; Fujiwara, A.
    Purification and properties of NADPH-linked L-sorbose reductase from Gluconobacter melanogenus N44-1 (1991), Agric. Biol. Chem., 55, 2043-2049.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
additional information not inhibitory: sodium fluoroacetate, sodium fluoride, KCN, monoiodoacetate Gluconobacter oxydans
N-ethylmaleimide 0.97 mM, 81% residual activity Gluconobacter oxydans
Na2HAsO4 0.97 mM, 90% residual activity Gluconobacter oxydans
p-chloromercuribenzoate 0.19 mM, 43% residual activity Gluconobacter oxydans
Quinine 0.49 mM, complete loss of activity Gluconobacter oxydans
Sodium azide 0.97 mM, 90% residual activity Gluconobacter oxydans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.111
-
NADPH pH 7.0, 25°C Gluconobacter oxydans
7.3
-
D-mannitol pH 10.0, 25°C Gluconobacter oxydans
160
-
D-fructose pH 7.0, 25°C Gluconobacter oxydans
167
-
D-sorbitol pH 10.0, 25°C Gluconobacter oxydans
328
-
L-sorbose pH 7.0, 25°C Gluconobacter oxydans

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Gluconobacter oxydans 5829
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
58000
-
gel filtration Gluconobacter oxydans
60000
-
1 * 60000, SDS-PAGE Gluconobacter oxydans

Organism

Organism UniProt Comment Textmining
Gluconobacter oxydans
-
-
-
Gluconobacter oxydans N44-1
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Gluconobacter oxydans

Storage Stability

Storage Stability Organism
-20°C, 0.01 M potassium buffer, pH 7.0, stable for at least one month Gluconobacter oxydans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5-keto-D-fructose + NADPH 86.8% of the rate with L-sorbose Gluconobacter oxydans ? + NADP+
-
r
5-keto-D-fructose + NADPH 86.8% of the rate with L-sorbose Gluconobacter oxydans N44-1 ? + NADP+
-
r
D-fructose + NADPH
-
Gluconobacter oxydans D-mannitol + NADP+
-
?
D-fructose + NADPH
-
Gluconobacter oxydans N44-1 D-mannitol + NADP+
-
?
D-mannitol + NADP+ 134% of the rate with L-sorbose Gluconobacter oxydans L-fructose + NADPH + H+
-
r
D-mannitol + NADP+ 134% of the rate with L-sorbose Gluconobacter oxydans N44-1 L-fructose + NADPH + H+
-
r
D-sorbitol + NADP+ 134% of the rate with L-sorbose Gluconobacter oxydans L-sorbose + NADPH + H+
-
r
D-sorbitol + NADP+ 134% of the rate with L-sorbose Gluconobacter oxydans N44-1 L-sorbose + NADPH + H+
-
r
erythritol + NADP+ 65.3% of the rate with L-sorbose Gluconobacter oxydans ? + NADPH
-
r
erythritol + NADP+ 65.3% of the rate with L-sorbose Gluconobacter oxydans N44-1 ? + NADPH
-
r
L-fructose + NADPH 142% of the rate with L-sorbose Gluconobacter oxydans D-mannitol + NADP+
-
r
L-sorbose + NADPH + H+
-
Gluconobacter oxydans D-sorbitol + NADP+
-
r
ribulose + NADPH 2.4% of the rate with L-sorbose Gluconobacter oxydans ? + NADP+
-
r
tagatose + NADPH 0.8% of the rate with L-sorbose Gluconobacter oxydans ? + NADP+
-
r
xylulose + NADPH 25.7% of the rate with L-sorbose Gluconobacter oxydans ? + NADP+
-
r

Subunits

Subunits Comment Organism
monomer 1 * 60000, SDS-PAGE Gluconobacter oxydans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
Gluconobacter oxydans

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
pH 6.5-7.5, 30 min, stable Gluconobacter oxydans
55
-
stable up to, for 10 min Gluconobacter oxydans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
reduction of L-sorbose Gluconobacter oxydans
10 10.5 oxidation of D-sorbitol Gluconobacter oxydans

pH Stability

pH Stability pH Stability Maximum Comment Organism
6.5 7.5 30°C, stable for 30 min Gluconobacter oxydans

Cofactor

Cofactor Comment Organism Structure
NADP+
-
Gluconobacter oxydans
NADPH
-
Gluconobacter oxydans