Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | not inhibitory: sodium fluoroacetate, sodium fluoride, KCN, monoiodoacetate | Gluconobacter oxydans | |
N-ethylmaleimide | 0.97 mM, 81% residual activity | Gluconobacter oxydans | |
Na2HAsO4 | 0.97 mM, 90% residual activity | Gluconobacter oxydans | |
p-chloromercuribenzoate | 0.19 mM, 43% residual activity | Gluconobacter oxydans | |
Quinine | 0.49 mM, complete loss of activity | Gluconobacter oxydans | |
Sodium azide | 0.97 mM, 90% residual activity | Gluconobacter oxydans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.111 | - |
NADPH | pH 7.0, 25°C | Gluconobacter oxydans | |
7.3 | - |
D-mannitol | pH 10.0, 25°C | Gluconobacter oxydans | |
160 | - |
D-fructose | pH 7.0, 25°C | Gluconobacter oxydans | |
167 | - |
D-sorbitol | pH 10.0, 25°C | Gluconobacter oxydans | |
328 | - |
L-sorbose | pH 7.0, 25°C | Gluconobacter oxydans |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Gluconobacter oxydans | 5829 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
58000 | - |
gel filtration | Gluconobacter oxydans |
60000 | - |
1 * 60000, SDS-PAGE | Gluconobacter oxydans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Gluconobacter oxydans | - |
- |
- |
Gluconobacter oxydans N44-1 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Gluconobacter oxydans |
Storage Stability | Organism |
---|---|
-20°C, 0.01 M potassium buffer, pH 7.0, stable for at least one month | Gluconobacter oxydans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-keto-D-fructose + NADPH | 86.8% of the rate with L-sorbose | Gluconobacter oxydans | ? + NADP+ | - |
r | |
5-keto-D-fructose + NADPH | 86.8% of the rate with L-sorbose | Gluconobacter oxydans N44-1 | ? + NADP+ | - |
r | |
D-fructose + NADPH | - |
Gluconobacter oxydans | D-mannitol + NADP+ | - |
? | |
D-fructose + NADPH | - |
Gluconobacter oxydans N44-1 | D-mannitol + NADP+ | - |
? | |
D-mannitol + NADP+ | 134% of the rate with L-sorbose | Gluconobacter oxydans | L-fructose + NADPH + H+ | - |
r | |
D-mannitol + NADP+ | 134% of the rate with L-sorbose | Gluconobacter oxydans N44-1 | L-fructose + NADPH + H+ | - |
r | |
D-sorbitol + NADP+ | 134% of the rate with L-sorbose | Gluconobacter oxydans | L-sorbose + NADPH + H+ | - |
r | |
D-sorbitol + NADP+ | 134% of the rate with L-sorbose | Gluconobacter oxydans N44-1 | L-sorbose + NADPH + H+ | - |
r | |
erythritol + NADP+ | 65.3% of the rate with L-sorbose | Gluconobacter oxydans | ? + NADPH | - |
r | |
erythritol + NADP+ | 65.3% of the rate with L-sorbose | Gluconobacter oxydans N44-1 | ? + NADPH | - |
r | |
L-fructose + NADPH | 142% of the rate with L-sorbose | Gluconobacter oxydans | D-mannitol + NADP+ | - |
r | |
L-sorbose + NADPH + H+ | - |
Gluconobacter oxydans | D-sorbitol + NADP+ | - |
r | |
ribulose + NADPH | 2.4% of the rate with L-sorbose | Gluconobacter oxydans | ? + NADP+ | - |
r | |
tagatose + NADPH | 0.8% of the rate with L-sorbose | Gluconobacter oxydans | ? + NADP+ | - |
r | |
xylulose + NADPH | 25.7% of the rate with L-sorbose | Gluconobacter oxydans | ? + NADP+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 60000, SDS-PAGE | Gluconobacter oxydans |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Gluconobacter oxydans |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
pH 6.5-7.5, 30 min, stable | Gluconobacter oxydans |
55 | - |
stable up to, for 10 min | Gluconobacter oxydans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
reduction of L-sorbose | Gluconobacter oxydans |
10 | 10.5 | oxidation of D-sorbitol | Gluconobacter oxydans |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6.5 | 7.5 | 30°C, stable for 30 min | Gluconobacter oxydans |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Gluconobacter oxydans | |
NADPH | - |
Gluconobacter oxydans |