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Literature summary for 1.1.1.286 extracted from

  • Takahashi, K.; Nakanishi, F.; Tomita, T.; Akiyama, N.; Lassak, K.; Albers, S.V.; Kuzuyama, T.; Nishiyama, M.
    Characterization of two ?-decarboxylating dehydrogenases from Sulfolobus acidocaldarius (2016), Extremophiles, 20, 843-853.
    View publication on PubMed
Search for more literature for 1.1.1.286

Cloned(Commentary)

Cloned (Comment) Organism
gene saci_2375, DNA and amino acid sequence determination and analysis, recombinant expression in Escherichia coli strain BL21(DE3)Codon-Plus-RIL Sulfolobus acidocaldarius

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.011
-
 homoisocitrate pH 8.0, 60°C, recombinant enzyme Sulfolobus acidocaldarius
0.027
-
 isocitrate pH 8.0, 60°C, recombinant enzyme Sulfolobus acidocaldarius

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
homoisocitrate + NADP+ Sulfolobus acidocaldarius
-
 2-oxoadipate + CO2 + NADPH + H+
-
 r
homoisocitrate + NADP+ Sulfolobus acidocaldarius MW001
-
 2-oxoadipate + CO2 + NADPH + H+
-
 r
isocitrate + NADP+ Sulfolobus acidocaldarius
-
 2-oxoglutarate + CO2 + NADPH + H+
-
 r
isocitrate + NADP+ Sulfolobus acidocaldarius MW001
-
 2-oxoglutarate + CO2 + NADPH + H+
-
 r
additional information Sulfolobus acidocaldarius Saci_2375 exhibits distinct and similar activities for isocitrate and homoisocitrate, but no detectable activity for 3-isopropylmalate. Saci_2375 is a dual function enzyme serving as isocitrate-homoisocitrate dehydrogenase ?
-
 ?
additional information Sulfolobus acidocaldarius MW001 Saci_2375 exhibits distinct and similar activities for isocitrate and homoisocitrate, but no detectable activity for 3-isopropylmalate. Saci_2375 is a dual function enzyme serving as isocitrate-homoisocitrate dehydrogenase ?
-
 ?

Organism

Organism UniProt Comment Textmining
Sulfolobus acidocaldarius Q4J6C9
-
-
Sulfolobus acidocaldarius MW001 Q4J6C9
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme from Escherichia coli strain BL21(DE3)Codon-Plus-RIL b anion exchange chromatography, ammonium sulfate fractionation, hydropobic interaction chromatography, ultrafiltration, and gel filtration Sulfolobus acidocaldarius

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
homoisocitrate + NADP+
-
 Sulfolobus acidocaldarius 2-oxoadipate + CO2 + NADPH + H+
-
 r
homoisocitrate + NADP+
-
 Sulfolobus acidocaldarius MW001 2-oxoadipate + CO2 + NADPH + H+
-
 r
isocitrate + NADP+
-
 Sulfolobus acidocaldarius 2-oxoglutarate + CO2 + NADPH + H+
-
 r
isocitrate + NADP+
-
 Sulfolobus acidocaldarius MW001 2-oxoglutarate + CO2 + NADPH + H+
-
 r
additional information Saci_2375 exhibits distinct and similar activities for isocitrate and homoisocitrate, but no detectable activity for 3-isopropylmalate. Saci_2375 is a dual function enzyme serving as isocitrate-homoisocitrate dehydrogenase Sulfolobus acidocaldarius ?
-
 ?
additional information Saci_2375 exhibits distinct and similar activities for isocitrate and homoisocitrate, but no detectable activity for 3-isopropylmalate. Saci_2375 is a dual function enzyme serving as isocitrate-homoisocitrate dehydrogenase Sulfolobus acidocaldarius MW001 ?
-
 ?

Subunits

Subunits Comment Organism
tetramer dimer of dimers Sulfolobus acidocaldarius

Synonyms

Synonyms Comment Organism
isocitrate-homoisocitrate dehydrogenase
-
 Sulfolobus acidocaldarius
Saci_2375
-
 Sulfolobus acidocaldarius
Saci_2375 locus name Sulfolobus acidocaldarius

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
 assay at Sulfolobus acidocaldarius

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
37
-
 homoisocitrate pH 8.0, 60°C, recombinant enzyme Sulfolobus acidocaldarius
61
-
 isocitrate pH 8.0, 60°C, recombinant enzyme Sulfolobus acidocaldarius

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Sulfolobus acidocaldarius

Cofactor

Cofactor Comment Organism Structure
NADP+ Saci_2375 is an NADP+-dependent enzyme with the ability to function as ICDH, EC 1.1.1.42, and HICDH, EC 1.1.1.87 Sulfolobus acidocaldarius
NADPH Saci_2375 is an NADP+-dependent enzyme with the ability to function as ICDH, EC 1.1.1.42, and HICDH, EC 1.1.1.87 Sulfolobus acidocaldarius

General Information

General Information Comment Organism
physiological function the failure to isolate a knockout mutant of saci_2375 suggests the indispensable role of Saci_2375 in cell viability Sulfolobus acidocaldarius

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2259
-
 isocitrate pH 8.0, 60°C, recombinant enzyme Sulfolobus acidocaldarius
3364
-
 homoisocitrate pH 8.0, 60°C, recombinant enzyme Sulfolobus acidocaldarius