Cloned (Comment) | Organism |
---|---|
- |
Pyrococcus horikoshii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0164 | - |
isocitrate | 70°C, pH 7.8 | Pyrococcus horikoshii | |
0.0183 | - |
homoisocitrate | 70°C, pH 7.8 | Pyrococcus horikoshii | |
0.0771 | - |
NAD+ | 70°C, pH 7.8 | Pyrococcus horikoshii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrococcus horikoshii | - |
expression in Escherichia coli | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme expressed in Escherichia coli, purification of inclusion bodies requires N-laurylsarcosine | Pyrococcus horikoshii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
homoisocitrate + NAD+ | - |
Pyrococcus horikoshii | ? + NADH | - |
? | |
isocitrate + NAD+ | about 20times more efficient than homoisocitrate, NAD+ is preferred over NADP+ | Pyrococcus horikoshii | 2-oxoglutarate + CO2 + NADH + H+ | - |
? | |
isocitrate + NADP+ | NAD+ is preferred over NADP+ | Pyrococcus horikoshii | 2-oxoglutarate + CO2 + NADPH + H+ | - |
? | |
additional information | no substrate: 3-isopropylmalate | Pyrococcus horikoshii | ? | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
13.7 | - |
homoisocitrate | 70°C, pH 7.8 | Pyrococcus horikoshii | |
14.8 | - |
isocitrate | 70°C, pH 7.8 | Pyrococcus horikoshii | |
21.8 | - |
NAD+ | 70°C, pH 7.8 | Pyrococcus horikoshii |