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Literature summary for 1.1.1.284 extracted from

  • Allais, J.J.; Louktibi, A.; Baratti, J.
    Oxidation of methanol by the yeast, Pichia pastoris. Purification and properties of the formaldehyde dehydrogenase (1983), Agric. Biol. Chem., 47, 1509-1516.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
Cd2+ 1 mM, complete inhibition [Candida] boidinii
Cd2+ 1 mM, complete inhibition Komagataella pastoris
citrate 1 mM, 24% inhibition Komagataella pastoris
CN-
-
[Candida] boidinii
CN- 1 mM, 48% inhibition Komagataella pastoris
Cu2+
-
[Candida] boidinii
Cu2+ 1 mM, 76% inhibition Komagataella pastoris
EDTA 1 mM, 12% inhibition Komagataella pastoris
Hg2+
-
[Candida] boidinii
Hg2+ 1 mM, 90% inhibition Komagataella pastoris
NADH
-
[Candida] boidinii
NADH product inhibition Komagataella pastoris
NO2- 1 mM, 14% inhibition Komagataella pastoris
Zn2+
-
[Candida] boidinii
Zn2+ 1 mM, 28% inhibition Komagataella pastoris

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.24
-
NAD+
-
Komagataella pastoris
0.43
-
formaldehyde
-
Komagataella pastoris
0.48
-
glutathione
-
Komagataella pastoris

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
39000
-
2 * 39000, SDS-PAGE Komagataella pastoris
84000
-
gel filtration Komagataella pastoris

Organism

Organism UniProt Comment Textmining
Kloeckera sp.
-
-
-
Komagataella pastoris
-
-
-
[Candida] boidinii
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Komagataella pastoris

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
10.2
-
-
Komagataella pastoris

Storage Stability

Storage Stability Organism
4°C, in presence of glycerol, 70-80% recovery of activity after 7 days Komagataella pastoris

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
formaldehyde + glutathione + NAD+
-
Komagataella pastoris S-formylglutathione + NADH + H+
-
?
formaldehyde + glutathione + NAD+
-
[Candida] boidinii S-formylglutathione + NADH + H+
-
?
formaldehyde + glutathione + NAD+
-
Kloeckera sp. S-formylglutathione + NADH + H+
-
?
formaldehyde + glutathione + NAD+
-
Komagataella pastoris IFP 206 S-formylglutathione + NADH + H+
-
?

Subunits

Subunits Comment Organism
dimer
-
[Candida] boidinii
dimer 2 * 39000, SDS-PAGE Komagataella pastoris

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
Kloeckera sp.
35
-
-
[Candida] boidinii
47
-
-
Komagataella pastoris

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 65 20°C: about 60% of maximal activity, 65°C: about 40% of maximal activity Komagataella pastoris

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
35
-
10 min, 50% loss of activity Kloeckera sp.
52
-
10 min, 50% loss of activity Komagataella pastoris
52
-
10 min, 50% loss of activity [Candida] boidinii

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.233
-
glutathione
-
Komagataella pastoris
0.267
-
formaldehyde
-
Komagataella pastoris

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.9
-
-
Komagataella pastoris
8
-
-
Komagataella pastoris
8
-
-
[Candida] boidinii
8
-
-
Kloeckera sp.

pH Range

pH Minimum pH Maximum Comment Organism
6.4 9.3 50% of maximal activity at pH 6.4 and at pH 9.3 Komagataella pastoris

pH Stability

pH Stability pH Stability Maximum Comment Organism
6.1
-
37°C, 10 min, 50% loss of activity Komagataella pastoris
7
-
37°C, 10 min, about 20% loss of activity Komagataella pastoris
8.5
-
37°C, 10 min, about 30% loss of activity Komagataella pastoris
9.4
-
37°C, 10 min, about 50% loss of activity Komagataella pastoris

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Komagataella pastoris
NAD+
-
[Candida] boidinii
NAD+
-
Kloeckera sp.