Literature summary for 1.1.1.284 extracted from

Tagliani, A.; Rossi, J.; Marchand, C.H.; De Mia, M.; Tedesco, D.; Gurrieri, L.; Meloni, M.; Falini, G.; Trost, P.; Lemaire, S.D.; Fermani, S.; Zaffagnini, M.
Structural and functional insights into nitrosoglutathione reductase from Chlamydomonas reinhardtii (2021), Redox Biol., 38, 101806 .

Search for more literature for 1.1.1.284

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Chlamydomonas reinhardtii

Crystallization (Commentary)

Crystallization (Comment)	Organism
structure of GSNOR1 of both apo- and holoform, to 1.8 A and 2.3 A resolution, respectively	Chlamydomonas reinhardtii

Inhibitors

Inhibitors	Comment	Organism	Structure
methyl methanethiosulfonate	complete inactivation	Chlamydomonas reinhardtii
additional information	not inhibitory: N-ethylmaleimide	Chlamydomonas reinhardtii

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0143	-	NADH	pH 7.9, temperature not specified in the publication	Chlamydomonas reinhardtii
0.0249	-	S-nitrosoglutathione	pH 7.9, temperature not specified in the publication	Chlamydomonas reinhardtii

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Zn2+	the catalytic domain contains two zinc ions. One zinc ion (Zn402) may have a structural role and it is coordinated with a tetrahedral geometry by cysteine residues Cys100, 103, 106 and 114 in both apo- and holoforms . The second zinc ion (Zn401) lies in the active site and has a catalytic role as a Lewis acid, activating the functional group of the substrate	Chlamydomonas reinhardtii

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
96000	-	gel filtration	Chlamydomonas reinhardtii

Organism

Organism	UniProt	Comment	Textmining
Chlamydomonas reinhardtii	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant protein, purified by Ni2+ affinity chromatography	Chlamydomonas reinhardtii

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	no substrate: glutathione disulfide	Chlamydomonas reinhardtii	?	-	-
S-hydroxymethylglutathione + NAD+	-	Chlamydomonas reinhardtii	S-formylglutathione + NADH + H+	-	?
S-nitrosoglutathione + NADH + H+	-	Chlamydomonas reinhardtii	GSH + NAD+ + NO	-	?

Subunits

Subunits	Comment	Organism
dimer	2 * 40000, SDS-PAGE, 2 * 41500, MALDI-TOF	Chlamydomonas reinhardtii

Synonyms

Synonyms	Comment	Organism
GSNOR1	-	Chlamydomonas reinhardtii

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
25	50	30 min, no loss of activity	Chlamydomonas reinhardtii
65	-	rapid inactivation above	Chlamydomonas reinhardtii

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
26.6	-	S-nitrosoglutathione	pH 7.9, temperature not specified in the publication	Chlamydomonas reinhardtii
27	-	NADH	pH 7.9, temperature not specified in the publication	Chlamydomonas reinhardtii

Cofactor

Cofactor	Comment	Organism	Structure
additional information	almost no activity with NADPH	Chlamydomonas reinhardtii
NADH	-	Chlamydomonas reinhardtii

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
1070	-	S-nitrosoglutathione	pH 7.9, temperature not specified in the publication	Chlamydomonas reinhardtii
1860	-	NADH	pH 7.9, temperature not specified in the publication	Chlamydomonas reinhardtii

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Release 2023.1 (January 2023)