Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis, sequence comparison, recombinant overexpression of SoGSNOR in transgenic Nicotiana tabacum cv. NC89 seedlings using the agrobacterium tumefaciens strain LBA4404 transfection system, quantitative RT-PCR enzyme expression analysis | Spinacia oleracea |
Protein Variants | Comment | Organism |
---|---|---|
additional information | overexpression of SoGSNOR alleviates excess nitrate-induced oxidative stress in transgenic Nicotiana tabacum cv. NC89 seedlings, phenotype, overview. Overexpression SoGSNOR enhances the germination rate of transgenic tobacco seeds under nitrate stress, and overexpression of SoGSNOR results in lower NO and SNOs contents in the tobacco seedlings | Spinacia oleracea |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-nitrosoglutathione + NADH + H+ | Spinacia oleracea | - |
GSSG + ammonia + NAD+ | - |
ir |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Spinacia oleracea | A0A182BP76 | cv. chaoji | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Spinacia oleracea | - |
seedling | - |
Spinacia oleracea | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-nitrosoglutathione + NADH + H+ | - |
Spinacia oleracea | GSSG + ammonia + NAD+ | - |
ir |
Subunits | Comment | Organism |
---|---|---|
? | x * 40800, about, sequence calculation | Spinacia oleracea |
Synonyms | Comment | Organism |
---|---|---|
GSNOR | - |
Spinacia oleracea |
S-nitrosoglutathione reductase | - |
Spinacia oleracea |
SoGSNOR | - |
Spinacia oleracea |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Spinacia oleracea |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Spinacia oleracea | sequence calculation | - |
6.82 |
Organism | Comment | Expression |
---|---|---|
Spinacia oleracea | the transcript and protein level of SoGSNOR are reduced after excess nitrate treatment for 24 h. Addition of NO donor to the nitrate solution decreases the SoGSNOR expression, while supplementation inhibitor of nitrate reductase and nitric oxide synthase increases its expression | down |
Spinacia oleracea | supplementation inhibitor of nitrate reductase and nitric oxide synthase increases SoGSNOR expression | up |
General Information | Comment | Organism |
---|---|---|
malfunction | overexpression of SoGSNOR in tobacco increases the germination rate of transgenic seeds, compared to the wild-type under nitrate stress. Higher photosynthetic rate, transpiration rate, stomatal conductance, water use efficiency and expression level of some stress-related genes are detected in the transgenic seedlings compared to wild-type under nitrate stress. The transgenic tobacco seedlings have lower malondialdehyde content, reactive oxygen species (ROS) fluorescence, and higher activities and transcript level of superoxide dismutase, catalase, peroxidase under nitrate stress. SoGSNOR transgenic tobacco plants have lower nitrate reductase activity and protein level, higher GSNOR and non-symbiotic class 1 hemoglobin (nsHb) protein level than the wild-type plants, leading to lower NO accumulation and SNOs contents under nitrate stress. Overexpression of SoGSNOR increases nitrate stress tolerance of tobacco by regulating ROS and RNS metabolism | Spinacia oleracea |
physiological function | S-nitrosylation, the addition of nitric oxide (NO) moiety to a reactive cysteine thiol, to form an S-nitrosothiol (SNO), is a prototypic redox-based post-translational modification. S-nitrosoglutathione reductase (GSNOR) is the major regulator of total cellular SNO levels in plants. It plays a role in excess nitrate stress | Spinacia oleracea |