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Literature summary for 1.1.1.284 extracted from

  • Sanghani, P.C.; Davis, W.I.; Zhai, L.; Robinson, H.
    Structure-function relationships in human glutathione-dependent formaldehyde dehydrogenase. Role of Glu-67 and Arg-368 in the catalytic mechanism (2006), Biochemistry, 45, 4819-4830.
    View publication on PubMed
Search for more literature for 1.1.1.284

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
E67L mutant in which Glu67 is substituted with Leu Homo sapiens
R368L mutant in which Arg368 is substituted with Leu Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
dodecanoic acid
-
 Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0002
-
 NADH wild type enzyme, pH 7.5 Homo sapiens
0.0017
-
 S-hydroxymethylglutathione wild type enzyme, pH 7.5, 30°C Homo sapiens
0.0033
-
 NAD+ wild type enzyme Homo sapiens
0.0048
-
 S-nitrosoglutathione wild type enzyme, pH 7.5, 25°C Homo sapiens
0.0093
-
 NAD+ wild type enzyme, pH 10 Homo sapiens
0.011
-
 NADH mutant R368L, pH 7.5 Homo sapiens
0.0144
-
 NAD+ mutant R368L, pH 7.5 Homo sapiens
0.038
-
 S-hydroxymethylglutathione mutant R368L, pH 7.5, 30°C Homo sapiens
0.041
-
 12-Hydroxydodecanoic acid wild type enzyme, pH 10 Homo sapiens
0.054
-
 NAD+ mutant E67L, pH 10 Homo sapiens
0.116
-
 NAD+ mutant E67L Homo sapiens
0.16
-
 NAD+ mutant R368L, pH 10 Homo sapiens
0.16
-
 12-Hydroxydodecanoic acid mutant R368L, pH 10 Homo sapiens
0.16
-
 S-nitrosoglutathione mutant R368L, pH 7.5, 25°C Homo sapiens
0.68
-
 12-Hydroxydodecanoic acid mutant E67L, pH 10 Homo sapiens
2.65
-
 S-hydroxymethylglutathione mutant E67L, pH 7.5, 30°C Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
DE52 column chromatography, Q-Sepharose column chromatography and Sephacryl S-100HR column chromatography Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
12-hydroxydodecanoic acid + NAD+
-
 Homo sapiens ? + NADH
-
 ?
S-hydroxymethylglutathione + NAD+
-
 Homo sapiens S-formylglutathione + NADH + H+
-
 ?
S-nitrosoglutathione + NADH
-
 Homo sapiens GSH + NAD+ + NO
-
 ?

Synonyms

Synonyms Comment Organism
FDH
-
 Homo sapiens
Glutathione-dependent formaldehyde dehydrogenase
-
 Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.92
-
 NAD+ mutant E67L, pH 7.5 Homo sapiens
1.4
-
 S-hydroxymethylglutathione mutant E67L, pH 7.5, 30°C Homo sapiens
2.5
-
 NAD+ wild type enzyme, pH 7.5 Homo sapiens
2.5
-
 S-hydroxymethylglutathione wild type enzyme, pH 7.5, 30°C Homo sapiens
15.3
-
 S-nitrosoglutathione mutant R368L, pH 7.5, 25°C Homo sapiens
16.7
-
 NAD+ mutant R368L, pH 7.5 Homo sapiens
20
-
 S-hydroxymethylglutathione mutant R368L, pH 7.5, 30°C Homo sapiens
29.3
-
 S-nitrosoglutathione wild type enzyme, pH 7.5, 25°C Homo sapiens
34.5
-
 NADH wild type enzyme, pH 7.5 Homo sapiens
203.3
-
 S-nitrosoglutathione mutant R368L, pH 7.5, 25°C Homo sapiens
230
-
 NADH mutant R368L, pH 7.5 Homo sapiens

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Homo sapiens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.148
-
 dodecanoic acid wild type enzyme Homo sapiens
0.161
-
 dodecanoic acid mutant R368L Homo sapiens
0.617
-
 dodecanoic acid mutant E67L Homo sapiens