Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 cells | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
E67L | mutant in which Glu67 is substituted with Leu | Homo sapiens |
R368L | mutant in which Arg368 is substituted with Leu | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
dodecanoic acid | - |
Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0002 | - |
NADH | wild type enzyme, pH 7.5 | Homo sapiens | |
0.0017 | - |
S-hydroxymethylglutathione | wild type enzyme, pH 7.5, 30°C | Homo sapiens | |
0.0033 | - |
NAD+ | wild type enzyme | Homo sapiens | |
0.0048 | - |
S-nitrosoglutathione | wild type enzyme, pH 7.5, 25°C | Homo sapiens | |
0.0093 | - |
NAD+ | wild type enzyme, pH 10 | Homo sapiens | |
0.011 | - |
NADH | mutant R368L, pH 7.5 | Homo sapiens | |
0.0144 | - |
NAD+ | mutant R368L, pH 7.5 | Homo sapiens | |
0.038 | - |
S-hydroxymethylglutathione | mutant R368L, pH 7.5, 30°C | Homo sapiens | |
0.041 | - |
12-Hydroxydodecanoic acid | wild type enzyme, pH 10 | Homo sapiens | |
0.054 | - |
NAD+ | mutant E67L, pH 10 | Homo sapiens | |
0.116 | - |
NAD+ | mutant E67L | Homo sapiens | |
0.16 | - |
NAD+ | mutant R368L, pH 10 | Homo sapiens | |
0.16 | - |
12-Hydroxydodecanoic acid | mutant R368L, pH 10 | Homo sapiens | |
0.16 | - |
S-nitrosoglutathione | mutant R368L, pH 7.5, 25°C | Homo sapiens | |
0.68 | - |
12-Hydroxydodecanoic acid | mutant E67L, pH 10 | Homo sapiens | |
2.65 | - |
S-hydroxymethylglutathione | mutant E67L, pH 7.5, 30°C | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
DE52 column chromatography, Q-Sepharose column chromatography and Sephacryl S-100HR column chromatography | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
12-hydroxydodecanoic acid + NAD+ | - |
Homo sapiens | ? + NADH | - |
? | |
S-hydroxymethylglutathione + NAD+ | - |
Homo sapiens | S-formylglutathione + NADH + H+ | - |
? | |
S-nitrosoglutathione + NADH | - |
Homo sapiens | GSH + NAD+ + NO | - |
? |
Synonyms | Comment | Organism |
---|---|---|
FDH | - |
Homo sapiens |
Glutathione-dependent formaldehyde dehydrogenase | - |
Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.92 | - |
NAD+ | mutant E67L, pH 7.5 | Homo sapiens | |
1.4 | - |
S-hydroxymethylglutathione | mutant E67L, pH 7.5, 30°C | Homo sapiens | |
2.5 | - |
NAD+ | wild type enzyme, pH 7.5 | Homo sapiens | |
2.5 | - |
S-hydroxymethylglutathione | wild type enzyme, pH 7.5, 30°C | Homo sapiens | |
15.3 | - |
S-nitrosoglutathione | mutant R368L, pH 7.5, 25°C | Homo sapiens | |
16.7 | - |
NAD+ | mutant R368L, pH 7.5 | Homo sapiens | |
20 | - |
S-hydroxymethylglutathione | mutant R368L, pH 7.5, 30°C | Homo sapiens | |
29.3 | - |
S-nitrosoglutathione | wild type enzyme, pH 7.5, 25°C | Homo sapiens | |
34.5 | - |
NADH | wild type enzyme, pH 7.5 | Homo sapiens | |
203.3 | - |
S-nitrosoglutathione | mutant R368L, pH 7.5, 25°C | Homo sapiens | |
230 | - |
NADH | mutant R368L, pH 7.5 | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Homo sapiens |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.148 | - |
dodecanoic acid | wild type enzyme | Homo sapiens | |
0.161 | - |
dodecanoic acid | mutant R368L | Homo sapiens | |
0.617 | - |
dodecanoic acid | mutant E67L | Homo sapiens |