Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.1.1.284 extracted from

  • Schuette, H.; Flossdorf, J.; Sahm, H.; Kula, M.R.
    Purification and properties of formaldehyde dehydrogenase and formate dehydrogenase from Candida boidinii (1976), Eur. J. Biochem., 62, 151-160.
    View publication on PubMed

General Stability

General Stability Organism
inactivated by freezing and thawing [Candida] boidinii

Inhibitors

Inhibitors Comment Organism Structure
Cd2+ 1 mM, complete inhibition [Candida] boidinii
CN- 1 mM, 42% inhibition [Candida] boidinii
Cu2+ 1 mM, complete inhibition [Candida] boidinii
Hg2+ 1 mM, complete inhibition [Candida] boidinii
iodoacetate 1 mM, 15% inhibition [Candida] boidinii
PCMB 1 mM, complete inhibition [Candida] boidinii
Zn2+ 1 mM, 50% inhibition [Candida] boidinii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.09
-
NAD+
-
[Candida] boidinii
0.13
-
glutathione
-
[Candida] boidinii
0.25
-
formaldehyde
-
[Candida] boidinii
1.2
-
methylglyoxal
-
[Candida] boidinii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
2 * 40000, SDS-PAGE [Candida] boidinii
80000
-
equilibrium sedimentation [Candida] boidinii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
formaldehyde + glutathione + NAD+ [Candida] boidinii enzyme is found only in methanol-grown cells and is absent in cells grown on ethanol or glucose as carbon source S-formylglutathione + NADH + H+
-
?

Organism

Organism UniProt Comment Textmining
[Candida] boidinii
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
[Candida] boidinii

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
47.9
-
-
[Candida] boidinii

Storage Stability

Storage Stability Organism
50% glycerol, stable for a long time [Candida] boidinii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
formaldehyde + glutathione + NAD+
-
[Candida] boidinii S-formylglutathione + NADH + H+
-
?
formaldehyde + glutathione + NAD+ enzyme is found only in methanol-grown cells and is absent in cells grown on ethanol or glucose as carbon source [Candida] boidinii S-formylglutathione + NADH + H+
-
?
methylglyoxal + glutathione + NAD+
-
[Candida] boidinii S-pyruvylglutathione + NADH
-
?

Subunits

Subunits Comment Organism
dimer 2 * 40000, SDS-PAGE [Candida] boidinii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5 9
-
[Candida] boidinii

Cofactor

Cofactor Comment Organism Structure
NAD+
-
[Candida] boidinii