General Stability | Organism |
---|---|
one freezing at -20°C results in full inactivation | Homo sapiens |
overnight dialysis to remove mercaptoethanol destroys 60% of its original activity | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
AgNO3 | NADH protects more effectively than NAD+ | Homo sapiens | |
formaldehyde | - |
Homo sapiens | |
Hg2+ | NADH protects more effectively than NAD+ | Homo sapiens | |
iodoacetate | NADH protects more effectively than NAD+ | Homo sapiens | |
methylglyoxal | - |
Homo sapiens | |
NAD+ | inhibits reverse reaction with NAD+ or NADP+ | Homo sapiens | |
NADH | inhibits reaction with formaldehyde, glutathione and NAD+ | Homo sapiens | |
NADPH | inhibits reaction with formaldehyde, glutathione and NAD+ | Homo sapiens | |
S-formylglutathione | inhibits reaction with formaldehyde, glutathione and NAD+ | Homo sapiens | |
S-formylglutathione | product inhibition with S-hydroxymethylglutathione | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0042 | - |
NADH | pH 5.7 | Homo sapiens | |
0.0081 | - |
glutathione | pH 8.0 | Homo sapiens | |
0.009 | - |
NAD+ | pH 8.0 | Homo sapiens | |
0.0136 | - |
NAD+ | pH 5.7 | Homo sapiens | |
0.016 | - |
NADH | pH 8.0 | Homo sapiens | |
0.045 | - |
NADPH | pH 5.7 | Homo sapiens | |
0.28 | - |
S-formylglutathione | pH 5.7, with NADPH as cofactor | Homo sapiens | |
0.3 | - |
S-formylglutathione | pH 5.7, with NADH as cofactor | Homo sapiens | |
0.57 | - |
NADP+ | pH 5.7 | Homo sapiens | |
4 | - |
pyruvylglutathione | pH 5.7, with NADH as cofactor | Homo sapiens | |
9.5 | - |
NADP+ | pH 8.0 | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Homo sapiens | 5829 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
81400 | - |
gel filtration | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Homo sapiens | - |
- |
- |
Mus musculus | - |
- |
- |
Oryctolagus cuniculus | - |
- |
- |
Ovis aries | - |
- |
- |
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
adrenal gland | - |
Bos taurus | - |
ascites tumor cell | - |
Mus musculus | - |
brain | - |
Homo sapiens | - |
brain | - |
Rattus norvegicus | - |
brain | - |
Bos taurus | - |
brain | - |
Oryctolagus cuniculus | - |
cardiac muscle | - |
Rattus norvegicus | - |
kidney | - |
Rattus norvegicus | - |
liver | - |
Mus musculus | - |
liver | - |
Homo sapiens | - |
liver | - |
Rattus norvegicus | - |
liver | - |
Bos taurus | - |
liver | - |
Oryctolagus cuniculus | - |
liver | - |
Ovis aries | - |
skeletal muscle | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
3.2 | - |
- |
Homo sapiens |
Storage Stability | Organism |
---|---|
-70°C, in presence of 2 mM mercaptoethanol, 50% loss of activity | Homo sapiens |
0°C, 50% loss of activity after 1 month | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-ethoxy-2-hydroxybutyraldehyde + glutathione + NAD+ | - |
Homo sapiens | S-(3-ethoxy-2-hydroxybutyryl)-glutathione + NADH + H+ | - |
? | |
formaldehyde + glutathione + NAD+ | - |
Mus musculus | S-formylglutathione + NADH + H+ | - |
? | |
formaldehyde + glutathione + NAD+ | - |
Homo sapiens | S-formylglutathione + NADH + H+ | - |
r | |
formaldehyde + glutathione + NAD+ | - |
Rattus norvegicus | S-formylglutathione + NADH + H+ | - |
r | |
formaldehyde + glutathione + NAD+ | - |
Bos taurus | S-formylglutathione + NADH + H+ | - |
? | |
formaldehyde + glutathione + NAD+ | - |
Oryctolagus cuniculus | S-formylglutathione + NADH + H+ | - |
? | |
formaldehyde + glutathione + NAD+ | - |
Ovis aries | S-formylglutathione + NADH + H+ | - |
? | |
formaldehyde + glutathione + NADP+ | - |
Homo sapiens | S-formylglutathione + NADPH + H+ | - |
r | |
formaldehyde + glutathione + NADP+ | - |
Rattus norvegicus | S-formylglutathione + NADPH + H+ | - |
r | |
glyoxal + glutathione + NAD+ | - |
Homo sapiens | S-oxoacetylglutathione + NADH | - |
? | |
hydroxypyruvaldehyde + glutathione + NAD+ | - |
Homo sapiens | S-hydroxypyruvylglutathione + NADH | - |
? | |
methylglyoxal + glutathione + NAD+ | - |
Homo sapiens | S-pyruvylglutathione + NADH | - |
? | |
methylglyoxal + glutathione + NAD+ | - |
Bos taurus | S-pyruvylglutathione + NADH | - |
? | |
pyruvylglutathione + NADH + H+ | - |
Homo sapiens | methylglyoxal + NAD+ | - |
r | |
S-formylglutathione + NADH | - |
Homo sapiens | formaldehyde + glutathione + NAD+ | - |
r | |
S-formylglutathione + NADH | - |
Rattus norvegicus | formaldehyde + glutathione + NAD+ | - |
r | |
S-formylglutathione + NADPH | - |
Homo sapiens | formaldehyde + glutathione + NADP+ | - |
r |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
15 min, without NADH, 40% loss of activity | Homo sapiens |
64 | - |
15 min, in presence of NADH, 40% loss of activity | Homo sapiens |
68 | - |
15 min, in presence of NADH, 80% loss of activity | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.7 | - |
reaction with S-formylglutathione and NADH | Homo sapiens |
6 | - |
reaction with NADP+ as cofactor | Homo sapiens |
8 | - |
reaction with NAD+ as cofactor | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Mus musculus | |
NAD+ | - |
Homo sapiens | |
NAD+ | - |
Rattus norvegicus | |
NAD+ | - |
Bos taurus | |
NAD+ | - |
Oryctolagus cuniculus | |
NAD+ | - |
Ovis aries | |
NADH | - |
Homo sapiens | |
NADPH | - |
Homo sapiens |