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Literature summary for 1.1.1.284 extracted from

  • Uotila, L.; Koivusalo, M.
    Formaldehyde dehydrogenase (1981), Methods Enzymol., 77, 314-320.
    View publication on PubMed

General Stability

General Stability Organism
NAD+ and NADH protect from denaturation by high temperatures, acid pH and storage at 0°C Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
ADP-ribose
-
Homo sapiens
Alpha-NAD+
-
Homo sapiens
Hg2+
-
Homo sapiens
NAD+ competitive, nonlinear inhibitor, when the concentration of NADH varies at constant S-formylglutathione concentration, noncompetitive inhibitor when the concentration of S-formylglutathione is varied at constant NADH concentration Homo sapiens
p-hydroxymercuribenzoate
-
Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0042
-
NADH pH 5.7 Homo sapiens
0.008
-
glutathione pH 8 Homo sapiens
0.009
-
formaldehyde pH 8 Homo sapiens
0.045
-
NADPH pH 5.7 Homo sapiens
0.3
-
S-formylglutathione pH 5.7, with NADH as cofactor Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.17
-
-
Homo sapiens

Storage Stability

Storage Stability Organism
0°C, half-life in absence of a stabilizer is a few h, in presence of 1 mM NAD+ or 40% v/v glycerol the half life is about 1 month, 0.5 mM NADH is even more effective Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-ethoxy-2-hydroxybutyraldehyde + glutathione + NAD+ weak activity Homo sapiens S-(3-ethoxy-2-hydroxybutyryl)-glutathione + NADH + H+
-
?
formaldehyde + glutathione + NAD+ at pH 8 the rate of the reverse reaction with S-formylglutathione and NADH is about the same as that of the forward reaction with formaldehyde, glutathione and NAD+. At pH 5.7 the rate of the reverse reaction with S-formylglutathione and NADH is 3.9times and with S-formylglutathione and NADPH 2.0times, that of the forward reaction rate with NAD+ at pH 8.0 Homo sapiens S-formylglutathione + NADH + H+
-
?
formaldehyde + glutathione + NAD+ at pH 8 the rate of the reverse reaction with S-formylglutathione and NADH is about the same as that of the forward reaction with formaldehyde, glutathione and NAD+. At pH 5.7 the rate of the reverse reaction with S-formylglutathione and NADH is 3.9times and with S-formylglutathione and NADPH 2.0times, that of the forward reaction rate with NAD+ at pH 8.0 Homo sapiens S-formylglutathione + NADH + H+
-
r
formaldehyde + glutathione + NADP+
-
Homo sapiens S-formylglutathione + NADPH + H+
-
r
glyoxal + glutathione + NAD+ weak activity Homo sapiens S-oxoacetylglutathione + NADH
-
?
hydroxypyruvaldehyde + glutathione + NAD+ weak activity Homo sapiens S-hydroxypyruvylglutathione + NADH
-
?
methylglyoxal + glutathione + NAD+ 85% of the activity with formaldehyde Homo sapiens S-pyruvylglutathione + NADH
-
?
S-formylglutathione + NADH
-
Homo sapiens formaldehyde + glutathione + NAD+
-
r
S-formylglutathione + NADPH
-
Homo sapiens formaldehyde + glutathione + NADP+
-
r
S-pyruvylglutathione + NADH
-
Homo sapiens methylglyoxal + glutathione + NAD+
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.7
-
reaction with S-formylglutathione and NADH Homo sapiens
8
-
oxidation of formaldehyde Homo sapiens

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Homo sapiens
NADH
-
Homo sapiens
NADP+ at pH 8 the Km-value is about 1000times greater than that for NAD+. At pH 6 NADP+ is used better but this reaction appears to be without physiological significance Homo sapiens
NADPH
-
Homo sapiens