Cloned (Comment) | Organism |
---|---|
recombinant expression of His6-tagged isozymes in Escherichia coli strain M15 | Populus trichocarpa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Michaelis-Menten kinetics | Populus trichocarpa | |
0.321 | - |
L-quinate | with NAD+, isozyme Poptr3, pH 8.5, 22°C | Populus trichocarpa | |
0.334 | - |
L-quinate | with NAD+, isozyme Poptr2, pH 8.5, 22°C | Populus trichocarpa | |
0.392 | - |
shikimate | with NAD+, isozyme Poptr3, pH 8.5, 22°C | Populus trichocarpa | |
0.833 | - |
shikimate | with NAD+, isozyme Poptr2, pH 8.5, 22°C | Populus trichocarpa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-quinate + NAD+ | Populus trichocarpa | - |
3-dehydroquinate + NADH + H+ | - |
? | |
additional information | Populus trichocarpa | the enzyme preferentially uses quinate as a substrate in vitro like a quinate dehydrogenase, EC 1.1.1.24, with only residual shikimate dehydrogenase, SDH, activity, cf. EC 1.1.1.25 | ? | - |
? | |
shikimate + NAD+ | Populus trichocarpa | - |
3-dehydroshikimate + NADH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Populus trichocarpa | - |
trees grown in a field at the University of Victoria | - |
Populus trichocarpa Nisqually-1 | - |
trees grown in a field at the University of Victoria | - |
Purification (Comment) | Organism |
---|---|
recombinant His6-tagged isozymes from Escherichia coli strain M15 by nickel affinity chromatography | Populus trichocarpa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
bark | Poptr3 and Poptr4 | Populus trichocarpa | - |
leaf | Poptr4 | Populus trichocarpa | - |
additional information | Poptr4 shows a distinct expression pattern with predominant expression in leaves, seedlings, and stomata and some expression in bark and differentiating tissues. Isozyme expression profiles, overview | Populus trichocarpa | - |
root | Poptr2 and Poptr3 are highly expressed in roots and root tips, respectively | Populus trichocarpa | - |
seedling | Poptr4 | Populus trichocarpa | - |
stem | Poptr3 is highly expressed in bark and some vascular tissues | Populus trichocarpa | - |
stoma | Poptr4 | Populus trichocarpa | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-quinate + NAD+ | - |
Populus trichocarpa | 3-dehydroquinate + NADH + H+ | - |
? | |
additional information | the enzyme preferentially uses quinate as a substrate in vitro like a quinate dehydrogenase, EC 1.1.1.24, with only residual shikimate dehydrogenase, SDH, activity, cf. EC 1.1.1.25 | Populus trichocarpa | ? | - |
? | |
shikimate + NAD+ | - |
Populus trichocarpa | 3-dehydroshikimate + NADH + H+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Poptr2 | - |
Populus trichocarpa |
Poptr3 | - |
Populus trichocarpa |
Poptr4 | - |
Populus trichocarpa |
SDH/QDH | - |
Populus trichocarpa |
shikimate/quinate dehydrogenase | - |
Populus trichocarpa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
assay at room temperature | Populus trichocarpa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | 9.5 | - |
Populus trichocarpa |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | no activity with NADP+ | Populus trichocarpa | |
NAD+ | - |
Populus trichocarpa |
General Information | Comment | Organism |
---|---|---|
evolution | members of the same gene family encode enzymes with either shikimate or quinate dehydrogenase activity. Plant SDHs are generally more similar to bacterial SDH/QDH YdiB (25-30% similarity) than to bacterial SDH AroE (21-28%) | Populus trichocarpa |
metabolism | reactions comprising the shikimate/quinate cycle, overview | Populus trichocarpa |
additional information | the enzymes have Gly residues instead of Ser residues in the active sites. The Ser-to-Gly conversion ompared to SDHs may generate extra space in the inferred Poptr isozymes active sites that can accommodate the hydroxyl group at the C1 position of quinate | Populus trichocarpa |
physiological function | quinate and its derivatives are protective secondary metabolites, quinate is an astringent feeding deterrent that can be formed in a single step reaction from 3-dehydroquinate catalyzed by quinate dehydrogenase | Populus trichocarpa |