KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.15 | - |
beta-NAD+ | pH 10, cosubstrate (-)-quinate | Rhodococcus rhodochrous | |
0.48 | - |
nicotinamide hypoxanthine dinucleotide | pH 10, cosubstrate (-)-quinate | Rhodococcus rhodochrous | |
0.51 | - |
oxidized nicotinamide 1,N6-ethanoadenine dinucleotide | pH 10, cosubstrate (-)-quinate | Rhodococcus rhodochrous | |
0.65 | - |
3-acetylpyridine adenine dinucleotide | pH 10, cosubstrate (-)-quinate | Rhodococcus rhodochrous | |
2.47 | - |
t-3,t-4-dihydroxycyclohexane-c-1-carboxylate | pH 10, (-)-enantiomer, cosubstrate NAD+ | Rhodococcus rhodochrous | |
2.56 | - |
dihydroshikimate | pH 10, (-)-enantiomer, cosubstrate NAD+ | Rhodococcus rhodochrous | |
2.95 | - |
L-quinate | pH 10, (-)-enantiomer, cosubstrate NAD+ | Rhodococcus rhodochrous | |
5.25 | - |
shikimate | pH 10, (-)-enantiomer, cosubstrate NAD+ | Rhodococcus rhodochrous |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
31500 | - |
1 * 31500, SDS-PAGE | Rhodococcus rhodochrous |
44000 | - |
gel filtration | Rhodococcus rhodochrous |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Rhodococcus rhodochrous | initial enzyme of the hydroaromatic pathway | ? | - |
? | |
additional information | Rhodococcus rhodochrous N75 | initial enzyme of the hydroaromatic pathway | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodococcus rhodochrous | - |
- |
- |
Rhodococcus rhodochrous N75 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
188fold | Rhodococcus rhodochrous |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
L-quinate + NAD(P)+ = 3-dehydroquinate + NAD(P)H + H+ | stereospecificity | Rhodococcus rhodochrous | |
shikimate + NAD(P)+ = 3-dehydroshikimate + NAD(P)H + H+ | stereospecificity | Rhodococcus rhodochrous |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Rhodococcus rhodochrous |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dihydroshikimate + NAD+ | highly stereospecific with regard to hydroaromatic substrates, oxidizes only the axial hydroxyl group at C-3 of the (-)-enantiomer, 38% of the activity with (-)-quinate | Rhodococcus rhodochrous | (1S,3R,4S)-3,4-dihydroxy-5-oxocyclohexanecarboxylic acid + NADH + H+ | (-)-enantiomer, reverse reaction: 3.3fold higher activity than with (-)-3-dehydroquinate | r | |
dihydroshikimate + NAD+ | highly stereospecific with regard to hydroaromatic substrates, oxidizes only the axial hydroxyl group at C-3 of the (-)-enantiomer, 38% of the activity with (-)-quinate | Rhodococcus rhodochrous N75 | (1S,3R,4S)-3,4-dihydroxy-5-oxocyclohexanecarboxylic acid + NADH + H+ | (-)-enantiomer, reverse reaction: 3.3fold higher activity than with (-)-3-dehydroquinate | r | |
L-quinate + 3-acetylpyridine adenine dinucleotide | 67% of the activity with NAD+ | Rhodococcus rhodochrous | 3-dehydroquinate + ? | - |
? | |
L-quinate + 3-acetylpyridine adenine dinucleotide | 67% of the activity with NAD+ | Rhodococcus rhodochrous N75 | 3-dehydroquinate + ? | - |
? | |
L-quinate + beta-NAD+ | highly stereospecific with regard to hydroaromatic substrates, oxidizes only the axial hydroxyl group at C-3 of the (-)-enantiomer, a single enzyme with both quinate and shikimate dehydrogenase activity | Rhodococcus rhodochrous | 3-dehydroquinate + beta-NADH + H+ | (-)-enantiomer, reverse reaction: lower activity than with t-4,c-5-dihydroxy-3-oxocyclohexane-c-1-carboxylate or 4-hydroxy-3-oxocyclohexane-c-1-carboxylate | r | |
L-quinate + beta-NAD+ | highly stereospecific with regard to hydroaromatic substrates, oxidizes only the axial hydroxyl group at C-3 of the (-)-enantiomer, a single enzyme with both quinate and shikimate dehydrogenase activity | Rhodococcus rhodochrous N75 | 3-dehydroquinate + beta-NADH + H+ | (-)-enantiomer, reverse reaction: lower activity than with t-4,c-5-dihydroxy-3-oxocyclohexane-c-1-carboxylate or 4-hydroxy-3-oxocyclohexane-c-1-carboxylate | r | |
L-quinate + NADP+ | 0.3% of the activity with NAD+ | Rhodococcus rhodochrous | 3-dehydroquinate + NADPH + H+ | - |
? | |
L-quinate + nicotinamide 1,N6-ethenoadenine dinucleotide | 69% of the activity with NAD+ | Rhodococcus rhodochrous | 3-dehydroquinate + ? | - |
? | |
L-quinate + nicotinamide hypoxanthine dinucleotide | 1.3fold higher activity than with NAD+ | Rhodococcus rhodochrous | 3-dehydroquinate + ? | - |
? | |
L-quinate + oxidized nicotinamide 1,N6-ethanoadenine dinucleotide | 69% activity compared to NAD+ | Rhodococcus rhodochrous | 3-dehydroquinate + reduced nicotinamide 1,N6-ethanoadenine dinucleotide | - |
r | |
additional information | initial enzyme of the hydroaromatic pathway | Rhodococcus rhodochrous | ? | - |
? | |
additional information | substrate specificity, enzyme is highly stereospecific with regard to hydroaromatic substrates, oxidizing only the axial hydroxyl group at C-3 of (-)-enantiomer of quinate, shikimate, dihydroshikimate and t-3,t-4-dihydroxycyclohexane-c-1-carboxylate, enzyme shows activity with several NAD+ analogues, reverse reaction: not 4-hydroxy-3-oxocyclohex-4-ene-c-1-carboxylate, not: alpha-NAD+, beta-NMN or nicotinic acid dinucleotide | Rhodococcus rhodochrous | ? | - |
? | |
additional information | initial enzyme of the hydroaromatic pathway | Rhodococcus rhodochrous N75 | ? | - |
? | |
additional information | substrate specificity, enzyme is highly stereospecific with regard to hydroaromatic substrates, oxidizing only the axial hydroxyl group at C-3 of (-)-enantiomer of quinate, shikimate, dihydroshikimate and t-3,t-4-dihydroxycyclohexane-c-1-carboxylate, enzyme shows activity with several NAD+ analogues, reverse reaction: not 4-hydroxy-3-oxocyclohex-4-ene-c-1-carboxylate, not: alpha-NAD+, beta-NMN or nicotinic acid dinucleotide | Rhodococcus rhodochrous N75 | ? | - |
? | |
shikimate + NAD+ | highly stereospecific with regard to hydroaromatic substrates, oxidizes only the axial hydroxyl group at C-3 of the (-)-enantiomer, a single enzyme with both quinate and shikimate dehydrogenase activity, 72% of the activity with (-)-quinate | Rhodococcus rhodochrous | 3-dehydroshikimate + NADH + H+ | (-)-enantiomer, reverse reaction: 15% of the activity with (-)-3-dehydroquinate | r | |
t-3,t-4-dihydroxycyclohexane-c-1-carboxylate + NAD+ | highly stereospecific with regard to hydroaromatic substrates, oxidizes only the axial hydroxyl group at C-3 of the (-)-enantiomer, 44% of the activity with (-)-quinate | Rhodococcus rhodochrous | 4-hydroxy-3-oxocyclohexane-c-1-carboxylate + NADH + H+ | (-)-isomer, reverse reaction: 2.2fold higher activity than with (-)-3-dehydroquinate | r | |
t-3-hydroxy-4-oxocyclohexane-c-1-carboxylate + NAD+ | 6% of the activity with (-)-quinate | Rhodococcus rhodochrous | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 31500, SDS-PAGE | Rhodococcus rhodochrous |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
t1/2: 1.85 min | Rhodococcus rhodochrous |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
reduction reaction, assay at | Rhodococcus rhodochrous |
10 | - |
oxidation reaction, quinate or shikimate as substrates, 50 mM glycine-KOH | Rhodococcus rhodochrous |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 8.5 | the rate of activity decreases more rapidly, maximal 25% at pH 7.5, at pH values from 8.5 to 7 when shikimate rather than quinate is used as substrate | Rhodococcus rhodochrous |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
3-acetylpyridine adenine dinucleotide | 67% of the activity with NAD+ | Rhodococcus rhodochrous | |
additional information | absolute requirement for a nicotinamide nucleotide cofactor for the oxidation of quinate or shikimate, cofactor specificity, not: alpha-NAD+, beta-NMN or nicotinic acid dinucleotide | Rhodococcus rhodochrous | |
NAD+ | - |
Rhodococcus rhodochrous | |
NADH | - |
Rhodococcus rhodochrous | |
NADP+ | 0.3% of the activity with NAD+ | Rhodococcus rhodochrous | |
nicotinamide 1,N6-ethenoadenine dinucleotide | 69% of the activity with NAD+ | Rhodococcus rhodochrous | |
nicotinamide hypoxanthine dinucleotide | 1.3fold higher activity than with NAD+ | Rhodococcus rhodochrous |