Cloned (Comment) | Organism |
---|---|
gene ldhD, phylogenetic analysis, recombinant expression of wwild-type and mutant enzymes in Escherichia coli strain BL21(DE3) | Sporolactobacillus inulinus |
Crystallization (Comment) | Organism |
---|---|
the complete tertiary structure of DLDH744 in complex with NAD+ is determined, PDB ID 4XKJ, determined by molecular replacement method and refined at 3.15 A-resolution | Sporolactobacillus inulinus |
Protein Variants | Comment | Organism |
---|---|---|
E263A | site-directed mutagenesis, the mutant shows highly reduced D-lactate dehydrogenase activity compared to the wild-type enzyme | Sporolactobacillus inulinus |
F298A | site-directed mutagenesis, almost inactive D-lactate dehydrogenase mutant | Sporolactobacillus inulinus |
G79A | site-directed mutagenesis, almost inactive D-lactate dehydrogenase mutant | Sporolactobacillus inulinus |
H295A | site-directed mutagenesis, inactive D-lactate dehydrogenase mutant | Sporolactobacillus inulinus |
M307A | site-directed mutagenesisthe mutant shows highly reduced D-lactate dehydrogenase activity compared to the wild-type enzyme | Sporolactobacillus inulinus |
additional information | glutamate dehydrogenase activities of enzyme mutants, overview | Sporolactobacillus inulinus |
R234A | site-directed mutagenesis, inactive D-lactate dehydrogenase mutant | Sporolactobacillus inulinus |
Y101A | site-directed mutagenesis, the mutant shows 50% reduced D-lactate dehydrogenase activity compared to the wild-type enzyme | Sporolactobacillus inulinus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.32 | - |
phenylpyruvate | recombinant enzyme, pH and temperature not specified in the publication | Sporolactobacillus inulinus | |
3.4 | - |
pyruvate | recombinant enzyme, pH and temperature not specified in the publication | Sporolactobacillus inulinus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sporolactobacillus inulinus | A0A0M3KL04 | - |
- |
Sporolactobacillus inulinus CASD | A0A0M3KL04 | - |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
(R)-lactate + NAD+ = pyruvate + NADH + H+ | catalytic mechanism | Sporolactobacillus inulinus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
7.5 | - |
purified recombinant enzyme, pH and temperature not specified in the publication | Sporolactobacillus inulinus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R)-lactate + NAD(P)+ | the enzyme is confirmed to be a typical D-lactate dehydrogenase based on the activity detected with substrate pyruvate and coenzyme NAD(P)H | Sporolactobacillus inulinus | pyruvate + NAD(P)H + H+ | - |
r | |
(R)-lactate + NAD(P)+ | the enzyme is confirmed to be a typical D-lactate dehydrogenase based on the activity detected with substrate pyruvate and coenzyme NAD(P)H | Sporolactobacillus inulinus CASD | pyruvate + NAD(P)H + H+ | - |
r | |
additional information | the bifunctional enzyme from Sporolactobacillus inulinus has both the D-LDH and NAD(P)+/NAD(P)H-utilizing glutamate dehydrogenase (GDH, EC 1.4.1.3) activities, the latter with reversible deamination. Identification of key residues from the crystal structure analysis and site-directed mutagenesis, enzyme reidues Arg234 and Gly79 residues play a significant role in both D-LDH and GDH activities. Residues His295 and Phe298 in DLDH744 are identified to be key residues for lactate dehydrogenase (LDH) activity only, whereas Tyr101 is a unique residue that is critical for GDH activity. DLDH744 is a strict D-2-hydroxyacid dehydrogenase, no formation of L-isomers | Sporolactobacillus inulinus | ? | - |
? | |
additional information | the bifunctional enzyme from Sporolactobacillus inulinus has both the D-LDH and NAD(P)+/NAD(P)H-utilizing glutamate dehydrogenase (GDH, EC 1.4.1.3) activities, the latter with reversible deamination. Identification of key residues from the crystal structure analysis and site-directed mutagenesis, enzyme reidues Arg234 and Gly79 residues play a significant role in both D-LDH and GDH activities. Residues His295 and Phe298 in DLDH744 are identified to be key residues for lactate dehydrogenase (LDH) activity only, whereas Tyr101 is a unique residue that is critical for GDH activity. DLDH744 is a strict D-2-hydroxyacid dehydrogenase, no formation of L-isomers | Sporolactobacillus inulinus CASD | ? | - |
? | |
phenylpyruvate + NADH + H+ | - |
Sporolactobacillus inulinus | D-phenyllactate + NAD+ | - |
r | |
phenylpyruvate + NADH + H+ | - |
Sporolactobacillus inulinus CASD | D-phenyllactate + NAD+ | - |
r | |
pyruvate + NAD(P)H + H+ | - |
Sporolactobacillus inulinus | (R)-lactate + NAD(P)+ | - |
r | |
pyruvate + NAD(P)H + H+ | - |
Sporolactobacillus inulinus CASD | (R)-lactate + NAD(P)+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
homodimer | two identical monomers of DLDH744 are associated at the cofactor binding domain to form the homodimer in one asymmetric unit. The dimer interface is stabilized mainly by hydrogen-bonding interactions between residues from alpha-helices, alpha5(Ser102-Arg119), alpa6(Leu121-Glu130), alpha12(Glu279-Arg285) and connecting loops, structure analysis, overview. His295 is supposed to function as the internal acid-base catalyst in dehydrogenase activity. Glu263 stabilizes the protonated form of His295, and Arg234 forms a hydrogen bond with the nicotinamide ribose ring | Sporolactobacillus inulinus |
Synonyms | Comment | Organism |
---|---|---|
D-LDH | - |
Sporolactobacillus inulinus |
DLDH744 | - |
Sporolactobacillus inulinus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
512.2 | - |
pyruvate | recombinant enzyme, pH and temperature not specified in the publication | Sporolactobacillus inulinus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Sporolactobacillus inulinus | |
NADH | - |
Sporolactobacillus inulinus | |
NADP+ | - |
Sporolactobacillus inulinus | |
NADPH | - |
Sporolactobacillus inulinus |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme belongs to the D-isomer-specific 2-hydroxyacid dehydrogenase family | Sporolactobacillus inulinus |