Application | Comment | Organism |
---|---|---|
synthesis | the polymers of lactic acid are used as biodegradable bioplastics. Polylactic acid, biodegradable polyester polymer of lactic acid, is mainly produced through a bacterial fermentation process | Lactobacillus jensenii |
Cloned (Comment) | Organism |
---|---|
recombinant expression of His-tagged selenomethionine-labeled enzyme in Escherichia coli strain B834 | Lactobacillus jensenii |
Crystallization (Comment) | Organism |
---|---|
purified recombinant selenomethionine-labeled enzyme, hanging drop vapour diffusion method, mixing of 0.0015 ml of 35 mg/ml protein in 40 mM Tris-HCl, pH 8.0, and 1 mM DTT with 0.0015 ml of reservoir solution containing 28% w/v PEG 400, 100 mM TrisHCl, pH 9.0, 200 mM magnesium sulfate at 22°C, X-ray diffraction structure determination and analysis at 2.0-2.1 A resolution by single wavelength anomalous dispersion using a selenomethionine derivative | Lactobacillus jensenii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lactobacillus jensenii | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged selenomethionine-labeled enzyme from Escherichia coli strain B834 by nickel affinity chromatography and gel filtration | Lactobacillus jensenii |
Synonyms | Comment | Organism |
---|---|---|
Ljd-LDH | - |
Lactobacillus jensenii |
General Information | Comment | Organism |
---|---|---|
physiological function | thermostable D-lactate dehydrogenase is a key enzyme for the production of the D-form of lactic acid from pyruvate concomitant with the oxidation of NADH to NAD+ | Lactobacillus jensenii |