Cloned (Comment) | Organism |
---|---|
D-LDH isozymes D-LDH1, D-LDH2, and D-LDH3, sequence comparisons and phylogenetic analysis | Lactobacillus jensenii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.04 | - |
NADH | isozyme D-LDH3, pH 8.0, 25°C | Lactobacillus jensenii | |
0.41 | - |
NAD+ | isozyme D-LDH3, pH 8.0, 25°C | Lactobacillus jensenii | |
0.7 | - |
NADH | isozyme D-LDH1, pH 8.0, 25°C | Lactobacillus jensenii | |
2.58 | - |
NAD+ | isozyme D-LDH2, pH 8.0, 25°C | Lactobacillus jensenii | |
2.75 | - |
NAD+ | isozyme D-LDH1, pH 8.0, 25°C | Lactobacillus jensenii | |
2.83 | - |
NADH | isozyme D-LDH2, pH 8.0, 25°C | Lactobacillus jensenii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R)-lactate + NAD+ | Lactobacillus jensenii | - |
pyruvate + NADH + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lactobacillus jensenii | - |
three isozymes, D-LDH1, D-LDH2, and D-LDH3 | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
7 | - |
isozyme D-LDH1, pH 8.0, 25°C, substrate D-phenylpyruvate | Lactobacillus jensenii |
9 | - |
isozyme D-LDH2, pH 8.0, 25°C, substrate D-phenylpyruvate | Lactobacillus jensenii |
18 | - |
isozyme D-LDH3, pH 8.0, 25°C, substrate oxaloacetic acid | Lactobacillus jensenii |
28 | - |
isozyme D-LDH3, pH 8.0, 25°C, substrate D-phenylpyruvate | Lactobacillus jensenii |
41 | - |
isozyme D-LDH2, pH 8.0, 25°C, substrate 2-oxoobutyric acid | Lactobacillus jensenii |
41.91 | - |
isozyme D-LDH3, pH 8.0, 25°C, substrate D-lactate | Lactobacillus jensenii |
68 | - |
isozyme D-LDH1, pH 8.0, 25°C, substrate 2-oxoobutyric acid | Lactobacillus jensenii |
88.71 | - |
isozyme D-LDH2, pH 8.0, 25°C, substrate D-lactate | Lactobacillus jensenii |
96.88 | - |
isozyme D-LDH1, pH 8.0, 25°C, substrate D-lactate | Lactobacillus jensenii |
189 | - |
isozyme D-LDH3, pH 8.0, 25°C, substrate 2-oxoobutyric acid | Lactobacillus jensenii |
348 | - |
isozyme D-LDH3, pH 8.0, 25°C, substrate pyruvate | Lactobacillus jensenii |
2877 | - |
isozyme D-LDH2, pH 8.0, 25°C, substrate oxaloacetic acid | Lactobacillus jensenii |
2997 | - |
isozyme D-LDH2, pH 8.0, 25°C, substrate pyruvate | Lactobacillus jensenii |
3151 | - |
isozyme D-LDH1, pH 8.0, 25°C, substrate oxaloacetic acid | Lactobacillus jensenii |
3838 | - |
isozyme D-LDH1, pH 8.0, 25°C, substrate pyruvate | Lactobacillus jensenii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R)-lactate + NAD+ | - |
Lactobacillus jensenii | pyruvate + NADH + H+ | - |
r | |
(R)-lactate + NAD+ | no activity with L-lactate | Lactobacillus jensenii | pyruvate + NADH + H+ | - |
r | |
2-oxobutyric acid + NADH + H+ | - |
Lactobacillus jensenii | (R)-2-hydroxybutyric acid + NAD+ | - |
ir | |
additional information | substrate specificities of the D-LDH isozymes, overview. (R)-2-Hydroxybutyric acid, D-(+)-malic acid, and D-(+)-3-phenyllactic acid are no substrates | Lactobacillus jensenii | ? | - |
? | |
oxaloacetic acid + NADH + H+ | - |
Lactobacillus jensenii | (R)-malate + NAD+ | - |
ir | |
phenylpyruvate + NADH + H+ | - |
Lactobacillus jensenii | D-phenyllactate + NAD+ | - |
ir |
Synonyms | Comment | Organism |
---|---|---|
D-LDH | - |
Lactobacillus jensenii |
D-LDH1 | - |
Lactobacillus jensenii |
D-LDH2 | - |
Lactobacillus jensenii |
D-LDH3 | - |
Lactobacillus jensenii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
isozymes D-LDH1 and D-LDH2 | Lactobacillus jensenii |
40 | - |
isozyme D-LDH3 | Lactobacillus jensenii |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
10 | 60 | isozyme D-LDH3, activity range, profile, overview | Lactobacillus jensenii |
10 | 55 | isozymes D-LDH1 and D-LDH2, activity range, profile, overview | Lactobacillus jensenii |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 55 | thermal stability of the D-LDH isozymes, pH 7.0, with sodium pyruvate as a substrate, profiles, overview | Lactobacillus jensenii |
40 | - |
all isozymes, stable up to | Lactobacillus jensenii |
50 | - |
isozyme D-LDH1, inactivation | Lactobacillus jensenii |
55 | - |
isozymes D-LDH2 and D-LDH3, inactivation | Lactobacillus jensenii |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.64 | - |
NAD+ | isozyme D-LDH3, pH 8.0, 25°C | Lactobacillus jensenii | |
3.65 | - |
NAD+ | isozyme D-LDH2, pH 8.0, 25°C | Lactobacillus jensenii | |
4.42 | - |
NAD+ | isozyme D-LDH1, pH 8.0, 25°C | Lactobacillus jensenii | |
8.28 | - |
NADH | isozyme D-LDH3, pH 8.0, 25°C | Lactobacillus jensenii | |
144.4 | - |
NADH | isozyme D-LDH1, pH 8.0, 25°C | Lactobacillus jensenii | |
516.4 | - |
NADH | isozyme D-LDH2, pH 8.0, 25°C | Lactobacillus jensenii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
isozymes D-LDH1 and D-LDH2 | Lactobacillus jensenii |
10 | - |
isozyme D-LDH3 | Lactobacillus jensenii |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 11 | isozyme D-LDH3, activity range, profile, overview | Lactobacillus jensenii |
7.5 | 11 | isozymes D-LDH1 and D-LDH2, activity range, profile, overview | Lactobacillus jensenii |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Lactobacillus jensenii | |
NADH | - |
Lactobacillus jensenii |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.41 | - |
NAD+ | isozyme D-LDH2, pH 8.0, 25°C | Lactobacillus jensenii | |
1.56 | - |
NAD+ | isozyme D-LDH3, pH 8.0, 25°C | Lactobacillus jensenii | |
1.61 | - |
NAD+ | isozyme D-LDH1, pH 8.0, 25°C | Lactobacillus jensenii | |
182.6 | - |
NADH | isozyme D-LDH2, pH 8.0, 25°C | Lactobacillus jensenii | |
206.3 | - |
NADH | isozyme D-LDH1, pH 8.0, 25°C | Lactobacillus jensenii | |
232.6 | - |
NADH | isozyme D-LDH3, pH 8.0, 25°C | Lactobacillus jensenii |