Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.1.1.274 extracted from

  • Ko, J.; Kim, I.; Yoo, S.; Min, B.; Kim, K.; Park, C.
    Conversion of methylglyoxal to acetol by Escherichia coli aldo-keto reductases (2005), J. Bacteriol., 187, 5782-5789.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.1.1.274

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.05
-
 methylglyoxal YqhE Escherichia coli
2.46
-
 methylglyoxal YafB Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
DEAE-Sepharose column chromatography and Superdex 200 gel filtration Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.01
-
 YafB, 1 mM 2-carboxybenzaldehyde as substrate Escherichia coli
0.01
-
 YafB, 10 mM D-xylose as substrate Escherichia coli
0.01
-
 YqhE, 1 mM 2-carboxybenzaldehyde as substrate Escherichia coli
0.0153
-
 YafB, 10 mM D-glucuronic acid as substrate Escherichia coli
0.0247
-
 YqhE, 10 mM D-glucuronic acid as substrate Escherichia coli
0.0401
-
 YafB, 1 mM 2,5-diketo-D-gluconate as substrate Escherichia coli
0.0424
-
 YafB, 0.1 mM 1,2-naphthoquinone as substrate Escherichia coli
0.0519
-
 YqhE, 1 mM 2,5-diketo-D-gluconate as substrate Escherichia coli
0.0949
-
 YqhE, 10 mM D-xylose as substrate Escherichia coli
0.127
-
 YqhE, 0.1 mM 1,2-naphthoquinone as substrate Escherichia coli
1.36
-
 YafB, 1 mM DL-glyceraldehyde as substrate Escherichia coli
2.21
-
 YqhE, 1 mM DL-glyceraldehyde as substrate Escherichia coli
2.23
-
 YafB, 1 mM 3-nitrobenzaldehyde as substrate Escherichia coli
2.5
-
 YqhE, 1 mM 3-nitrobenzaldehyde as substrate Escherichia coli
2.79
-
 YafB, 1 mM benzaldehyde as substrate Escherichia coli
3.88
-
 YqhE, 1 mM benzaldehyde as substrate Escherichia coli
6.53
-
 YafB, 1 mM 4-nitrobenzaldehyde as substrate Escherichia coli
10.3
-
 YqhE, 1 mM 4-nitrobenzaldehyde as substrate Escherichia coli
14.9
-
 YafB, 1 mM methylglyoxal as substrate Escherichia coli
21
-
 YqhE, 1 mM methylglyoxal as substrate Escherichia coli
39.1
-
 YafB, 1 mM phenylglyoxal as substrate Escherichia coli
64.4
-
 YqhE, 1 mM phenylglyoxal as substrate Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,2-naphthoquinone + NADPH
-
 Escherichia coli ? + NADP+
-
 ?
2,5-diketo-D-gluconate + NADPH
-
 Escherichia coli ? + NADP+
-
 ?
2-carboxybenzaldehyde + NADPH
-
 Escherichia coli 2-hydroxybenzoate + NADP+
-
 ?
3-nitrobenzaldehyde + NADPH
-
 Escherichia coli 3-nitrobenzyl alcohol + NADP+
-
 ?
4-nitrobenzaldehyde + NADPH
-
 Escherichia coli 4-nitrobenzyl alcohol + NADP+
-
 ?
benzaldehyde + NADPH
-
 Escherichia coli benzyl alcohol + NADP+
-
 ?
D-glucuronic acid + NADPH
-
 Escherichia coli ? + NADP+
-
 ?
D-xylose + NADPH
-
 Escherichia coli ? + NADP+
-
 ?
DL-glyceraldehyde + NADPH + H+
-
 Escherichia coli glycerol + NADP+
-
 ?
methylglyoxal + NADPH
-
 Escherichia coli ? + NADP+
-
 ?
phenylglyoxal + NADPH
-
 Escherichia coli ? + NADP+
-
 ?

Synonyms

Synonyms Comment Organism
2,5-diketo-gluconate reductase
-
 Escherichia coli
2,5-DKG
-
 Escherichia coli
YafB
-
 Escherichia coli
YqhE
-
 Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
27.62
-
 methylglyoxal YqhE Escherichia coli
29.15
-
 methylglyoxal YafB Escherichia coli
81
-
 methylglyoxal YafB Escherichia coli

Cofactor

Cofactor Comment Organism Structure
NADPH
-
 Escherichia coli