Application | Comment | Organism |
---|---|---|
synthesis | enzyme may be useful for the synthesis of the ascorbate precursor 2-keto-L-gulonate | Escherichia coli |
Cloned (Comment) | Organism |
---|---|
overexpression of isoenzymes A and B | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
29000 | - |
1 * 29000, isoenzymes A and B, SDS-PAGE | Escherichia coli |
30000 | - |
isoenzymes A and B, gel filtration | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P30863 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant isoenzymes A and B, affinity chitin-binding tag | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,5-didehydro-D-gluconate + NADPH | both isoenzymes A and B are specific for NADPH | Escherichia coli | 2-keto-L-gulonate + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 29000, isoenzymes A and B, SDS-PAGE | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
isoenzyme B | Escherichia coli |
7.5 | - |
isoenzyme A | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Escherichia coli | |
NADPH | - |
Escherichia coli |