Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.1.1.271 extracted from

  • Byun, S.G.; Kim, M.D.; Lee, W.H.; Lee, K.J.; Han, N.S.; Seo, J.H.
    Production of GDP-L-fucose, L-fucose donor for fucosyloligosaccharide synthesis, in recombinant Escherichia coli (2007), Appl. Microbiol. Biotechnol., 74, 768-775.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
isopropyl-beta-D-thioglucopyranoside
-
Escherichia coli K-12

Cloned(Commentary)

Cloned (Comment) Organism
gene wcaG, overexpression in strain BL21(DE3) together with GDP-D-mannose-4,6-dehydratase Escherichia coli K-12

Protein Variants

Protein Variants Comment Organism
additional information effective GDP-L-fucose production in recombinant Escherichia coli by expression of GDP-D-mannose-4,6-dehydratase and GDP-4-keto-6-deoxymannose 3,5-epimerase 4-reductase, method optimization at 25°C and 0.1 mM isopropyl-beta-D-thioglucopyranoside, overview Escherichia coli K-12

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
GDP-4-dehydro-6-deoxy-D-mannose + NADPH Escherichia coli K-12 the enzyme is crucial in the biosynthesis of GDP-L-fucose together with GDP-D-mannose-4,6-dehydratase, overview GDP-L-fucose + NADP+
-
r

Organism

Organism UniProt Comment Textmining
Escherichia coli K-12
-
gene wcaG
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Escherichia coli K-12

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GDP-4-dehydro-6-deoxy-D-mannose + NADPH
-
Escherichia coli K-12 GDP-L-fucose + NADP+
-
r
GDP-4-dehydro-6-deoxy-D-mannose + NADPH the enzyme is crucial in the biosynthesis of GDP-L-fucose together with GDP-D-mannose-4,6-dehydratase, overview Escherichia coli K-12 GDP-L-fucose + NADP+
-
r

Synonyms

Synonyms Comment Organism
GDP-4-keto-6-deoxymannose 3, 5-epimerase 4-reductase
-
Escherichia coli K-12
wcaG
-
Escherichia coli K-12

Cofactor

Cofactor Comment Organism Structure
NADP+
-
Escherichia coli K-12
NADPH dependent on Escherichia coli K-12