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Literature summary for 1.1.1.270 extracted from

  • Debieu, D.; Bach, J.; Montesinos, E.; Fillinger, S.; Leroux, P.
    Role of sterol 3-ketoreductase sensitivity in susceptibility to the fungicide fenhexamid in Botrytis cinerea and other phytopathogenic fungi (2013), Pest Manag. Sci., 69, 642-651.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
additional information erg27 mutation and erg27 polymorphism in different strains and fenhexamid phenotypes, overview Botrytis cinerea

Inhibitors

Inhibitors Comment Organism Structure
acetonitrile
-
Botrytis cinerea
dimethyl sulfoxide
-
Botrytis cinerea
ethanol
-
Botrytis cinerea
fenhexamid Erg27 mutations causing amino acid substitutions in or near the transmembrane domain strongly decrease the affinity of fenhexamid for 3-ketoreductase. Sterol 3-ketoreductase sensitivity to fenhexamid in the various fungal strains, overview Botrytis cinerea
propan-2-ol
-
Botrytis cinerea

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome
-
Botrytis cinerea
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Botrytis cinerea

Organism

Organism UniProt Comment Textmining
Botrytis cinerea
-
gene erg27
-

Source Tissue

Source Tissue Comment Organism Textmining
mycelium
-
Botrytis cinerea
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4alpha-methylfecosterone + NADPH + H+
-
Botrytis cinerea 4alpha-methylfecosterol + NADP+ 4alpha-methylfecosterol is further converted to ergosterol ?
fecosterone + NADPH + H+
-
Botrytis cinerea fecosterol + NADP+
-
?
additional information substrate for 3-ketoreductase in the first sterol C-4 demethylation cycle is 4alpha-methylfecosterone, whereas that for the second C-4 demethylation cycle is fecosterone Botrytis cinerea ?
-
?

Synonyms

Synonyms Comment Organism
sterol 3-ketoreductase
-
Botrytis cinerea

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Botrytis cinerea

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
assay at Botrytis cinerea

Cofactor

Cofactor Comment Organism Structure
NADPH
-
Botrytis cinerea

General Information

General Information Comment Organism
malfunction two Botrytis cinerea fenhexamid-resistant phenotypes both result from mutations in the erg27 gene encoding 3-ketoreductase. Erg27 mutations causing amino acid substitutions in or near the transmembrane domain strongly decrease the affinity of fenhexamid for 3-ketoreductase Botrytis cinerea
metabolism the 3-ketoreductase is involved at C-4 demethylation steps during ergosterol biosynthesis. Steryl compound contents of Botrytis strains with different fenhexamid susceptibilities, overview Botrytis cinerea